Repository logo
 
Publication

Multiparametric in vitro genotoxicity assessment of different variants of amorphous silica nanomaterials in rat alveolar epithelial cells

2023-12-01Journal article Open access
Simple item page
dc.contributor.authorBrandão, Fátima
dc.contributor.authorCosta, Carla
dc.contributor.authorBessa, Maria João
dc.contributor.authorValdiglesias, Vanessa
dc.contributor.authorHellack, Bryan
dc.contributor.authorHaase, Andrea
dc.contributor.authorFraga, Sónia
dc.contributor.authorTeixeira, João Paulo
dc.date.accessioned2024-02-02T14:04:33Z
dc.date.available2024-02-02T14:04:33Z
dc.date.issued2023-12-01
dc.description.abstractThe hazard posed to human health by inhaled amorphous silica nanomaterials (aSiO2 NM) remains uncertain. Herein, we assessed the cyto- and genotoxicity of aSiO2 NM variants covering different sizes (7, 15, and 40 nm) and surface modifications (unmodified, phosphonate-, amino- and trimethylsilyl-modified) on rat alveolar epithelial (RLE-6TN) cells. Cytotoxicity was evaluated at 24 h after exposure to the aSiO2 NM variants by the lactate dehydrogenase (LDH) release and WST-1 reduction assays, while genotoxicity was assessed using different endpoints: DNA damage (single- and double-strand breaks [SSB and DSB]) by the comet assay for all aSiO2 NM variants; cell cycle progression and γ-H2AX levels (DSB) by flow cytometry for those variants that presented higher cytotoxic and DNA damaging potential. The variants with higher surface area demonstrated a higher cytotoxic potential (SiO2_7, SiO2_15_Unmod, SiO2_15_Amino, and SiO2_15_Phospho). SiO2_40 was the only variant that induced significant DNA damage on RLE-6TN cells. On the other hand, all tested variants (SiO2_7, SiO2_15_Unmod, SiO2_15_Amino, and SiO2_40) significantly increased total γ-H2AX levels. At high concentrations (28 µg/cm2), a decrease in G0/G1 subpopulation was accompanied by a significant increase in S and G2/M sub-populations after exposure to all tested materials except for SiO2_40 which did not affect cell cycle progression. Based on the obtained data, the tested variants can be ranked for its genotoxic DNA damage potential as follows: SiO2_7 = SiO2_40 = SiO2_15_Unmod > SiO2_15_Amino. Our study supports the usefulness of multiparametric approaches to improve the understanding on NM mechanisms of action and hazard prediction.pt_PT
dc.description.sponsorshipThis work was supported by the Portuguese Foundation for Science and Technology (FCT) through ERA-NET SIINN project NanoToxClass (SIINN/0001/2013). This work was also supported by the NanoBioBarriers project (POCI-01-0145-FEDER-031162), co-financed by the Operational Program for Competitiveness and Internationalization (POCI) through European Regional Development Funds (FEDER/FNR); and by the Spanish Ministry of Science and Innovation: MCIN/AEI/10.13039/501100011033 (Grant PID2020-114908GA-I00); and the Ministry of Education, Culture and Sport BEAGAL18/00142 to V. Valdiglesias. F. Brandão (SFRH/BD/101060/2014) and M.J. Bessa (SFRH/BD/12046/2016) are recipients of FCT PhD scholarships. The Doctoral Program in Biomedical Sciences of the ICBAS – University of Porto offered additional funds. S. Fraga thanks FCT for funding through program DL 57/2016 – Norma transitória (Ref. DL-57/INSA-06/2018). Thanks are also due to FCT/MCTES for the financial support to EPIUnit (UIDB/04750/2020) and ITR (LA/P/0064/2020).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNanotoxicology. 2023 Aug-Sep;17(6-7):511-528. doi: 10.1080/17435390.2023.2265481. Epub 2023 Dec 1.pt_PT
dc.identifier.doi10.1080/17435390.2023.2265481pt_PT
dc.identifier.issn1743-5390
dc.identifier.urihttp://hdl.handle.net/10400.18/9029
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherTaylor and Francispt_PT
dc.relationEstablishing nanomaterial grouping/ classification strategies according to toxicity and biological effects for supporting risk assessment
dc.relationEstablishing grouping strategies according to toxicity and biological effects for silica based manufactured nanomaterials
dc.relationWater Circle – Dissemination of scientific knowledge as a prevention strategy in the domain of water preservation: from university research to secondary school students
dc.relationEpidemiology Research Unit - Institute of Public Health, University of Porto
dc.relationLaboratory for Integrative and Translational Research in Population Health
dc.relation.publisherversionhttps://www.tandfonline.com/doi/full/10.1080/17435390.2023.2265481pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectNanomaterialspt_PT
dc.subjectSilica Nanomaterialspt_PT
dc.subjectLungpt_PT
dc.subjectIn vitropt_PT
dc.subjectGenotoxicitypt_PT
dc.subjectNanotoxicologypt_PT
dc.subjectCytotoxicitypt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleMultiparametric in vitro genotoxicity assessment of different variants of amorphous silica nanomaterials in rat alveolar epithelial cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleEstablishing nanomaterial grouping/ classification strategies according to toxicity and biological effects for supporting risk assessment
oaire.awardTitleEstablishing grouping strategies according to toxicity and biological effects for silica based manufactured nanomaterials
oaire.awardTitleWater Circle – Dissemination of scientific knowledge as a prevention strategy in the domain of water preservation: from university research to secondary school students
oaire.awardTitleEpidemiology Research Unit - Institute of Public Health, University of Porto
oaire.awardTitleLaboratory for Integrative and Translational Research in Population Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/ERA-SIINN%2F0001%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FMED-TOX%2F31162%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F101060%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F112046%2F2015/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04750%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0064%2F2020/PT
oaire.citation.endPage528pt_PT
oaire.citation.issue6-7pt_PT
oaire.citation.startPage511pt_PT
oaire.citation.titleNanotoxicologypt_PT
oaire.citation.volume17pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream9471 - RIDTI
oaire.fundingStreamOE
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNamePinto Brandão
person.familyNameCosta
person.familyNameBessa
person.familyNameValdiglesias
person.familyNameHaase
person.familyNameFraga
person.familyNameRamos Teixeira
person.givenNameMaria de Fátima
person.givenNameCarla
person.givenNameMaria João
person.givenNameVanessa
person.givenNameAndrea
person.givenNameSónia
person.givenNameJoão Paulo
person.identifierD-8155-2013
person.identifierN-6576-2013
person.identifier.ciencia-id9B1C-958D-FA34
person.identifier.ciencia-id411A-17DD-91E1
person.identifier.ciencia-id7710-8F47-7A48
person.identifier.ciencia-id8C10-F04E-92FE
person.identifier.ciencia-id0717-20FD-1212
person.identifier.ciencia-id4F15-B322-59B4
person.identifier.orcid0000-0002-2933-8561
person.identifier.orcid0000-0002-0027-8462
person.identifier.orcid0000-0001-5357-6167
person.identifier.orcid0000-0002-5572-1089
person.identifier.orcid0000-0002-5288-7876
person.identifier.orcid0000-0001-9386-2336
person.identifier.orcid0000-0002-6679-5702
person.identifier.ridK-4048-2014
person.identifier.ridK-7728-2014
person.identifier.scopus-author-id55984029000
person.identifier.scopus-author-id16551208900
person.identifier.scopus-author-id7005283366
person.identifier.scopus-author-id57069567500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com a política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication97204514-7337-4fb4-98b5-d35253435e75
relation.isAuthorOfPublicationbf7218f4-7215-461a-b884-5ceed94e9111
relation.isAuthorOfPublicationff69cf1a-c213-403b-9cca-894e45142953
relation.isAuthorOfPublicationb35d9c6b-4095-4bc0-9929-c469a4fb0d37
relation.isAuthorOfPublication3ae25907-bcc4-4969-b420-7756da5d04d0
relation.isAuthorOfPublicationab130d77-047a-4459-aadc-16f22e40a1ce
relation.isAuthorOfPublicationbcd073ef-1e03-49cd-9dfa-df00cc26cf6b
relation.isAuthorOfPublication.latestForDiscovery3ae25907-bcc4-4969-b420-7756da5d04d0
relation.isProjectOfPublicationa118e28a-71df-4d9f-b18c-9d4e164baf22
relation.isProjectOfPublication1370de00-d228-47a7-8aed-320831dc4dcd
relation.isProjectOfPublication8bcb6d0f-fda0-470a-906d-c67c257beedb
relation.isProjectOfPublication6126e3d1-94a1-4294-9ca1-fc75d211b630
relation.isProjectOfPublication05f4f337-eeba-4c07-82ed-4202b21618a2
relation.isProjectOfPublication18ca3cb0-ef66-4dd8-b45e-4cb0654cd7e8
relation.isProjectOfPublication.latestForDiscoverya118e28a-71df-4d9f-b18c-9d4e164baf22

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Brandão et al. 2023.pdf
Size:
1.95 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

DSA - Artigos em revistas internacionais