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The Environmental Acinetobacter baumannii Isolate DSM30011 Reveals Clues into the Preantibiotic Era Genome Diversity, Virulence Potential, and Niche Range of a Predominant Nosocomial Pathogen

2017-09-01Journal article Open access
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dc.contributor.authorRepizo, Guillermo D.
dc.contributor.authorViale, Alejandro M.
dc.contributor.authorBorges, Vítor
dc.contributor.authorCameranesi, María M.
dc.contributor.authorTaib, Najwa
dc.contributor.authorEspariz, Martín
dc.contributor.authorBrochier-Armanet, Céline
dc.contributor.authorGomes, João Paulo
dc.contributor.authorSalcedo, Suzana P.
dc.date.accessioned2018-03-06T15:54:52Z
dc.date.available2018-03-06T15:54:52Z
dc.date.issued2017-09-01
dc.descriptionFree PMC Article: https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/28934377/pt_PT
dc.description.abstractAcinetobacter baumannii represents nowadays an important nosocomial opportunistic pathogen whose reservoirs outside the clinical setting are obscure. Here, we traced the origins of the collection strain A. baumannii DSM30011 to an isolate first reported in 1944, obtained from the enriched microbiota responsible of the aerobic decomposition of the resinous desert shrub guayule. Whole-genome sequencing and phylogenetic analysis based on core genes confirmed DSM30011 affiliation to A. baumannii. Comparative studies with 32 complete A. baumannii genomes revealed the presence of 12 unique accessory chromosomal regions in DSM30011 including five encompassing phage-related genes, five containing toxin genes of the type-6 secretion system, and one with an atypical CRISPRs/cas cluster. No antimicrobial resistance islands were identified in DSM30011 agreeing with a general antimicrobial susceptibility phenotype including folate synthesis inhibitors. The marginal ampicillin resistance of DSM30011 most likely derived from chromosomal ADC-type ampC and blaOXA-51-type genes. Searching for catabolic pathways genes revealed several clusters involved in the degradation of plant defenses including woody tissues and a previously unreported atu locus responsible of aliphatic terpenes degradation, thus suggesting that resinous plants may provide an effective niche for this organism. DSM30011 also harbored most genes and regulatory mechanisms linked to persistence and virulence in pathogenic Acinetobacter species. This strain thus revealed important clues into the genomic diversity, virulence potential, and niche ranges of the preantibiotic era A. baumannii population, and may provide an useful tool for our understanding of the processes that led to the recent evolution of this species toward an opportunistic pathogen of humans.pt_PT
dc.description.sponsorshipThis work was supported by a FINOVI Young Researcher Grant awarded to S.P.S.; by an Investissement d’Avenir (ANR-10-BINF-01-01) grant awarded to C.B.-A., and from grants to A.M.V. from the Agencia Nacional de Promocion Cientíıfica y Tecnologica (ANPCyT); Consejo Nacional de Investigaciones Cientıficas y Técnicas (CONICET-PIP1055); Ministerio de Ciencia, Tecnologıa e Innovacion Productiva, Provincia de Santa Fe; Argentina. A.M.V., G.D.R., and M.E. are staff members of CONICET. C.B.-A. is member of the Institut Universitaire de France.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGenome Biol Evol. 2017 Sep 1;9(9):2292-2307. doi: 10.1093/gbe/evx162.pt_PT
dc.identifier.doi10.1093/gbe/evx162pt_PT
dc.identifier.issn1759-6653 (online)
dc.identifier.urihttp://hdl.handle.net/10400.18/5218
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherOxford University Press/Society for Molecular Biology and Evolutionpt_PT
dc.relation.publisherversionhttps://academic.oup.com/gbe/article-lookup/doi/10.1093/gbe/evx162pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectAcinetobacter baumanniipt_PT
dc.subjectCRISPRs/caspt_PT
dc.subjectComparative Genomicspt_PT
dc.subjectPreantibiotic era bacteriumpt_PT
dc.subjectVirulence Factorspt_PT
dc.subjectInfecções Respiratóriaspt_PT
dc.titleThe Environmental Acinetobacter baumannii Isolate DSM30011 Reveals Clues into the Preantibiotic Era Genome Diversity, Virulence Potential, and Niche Range of a Predominant Nosocomial Pathogenpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage2307pt_PT
oaire.citation.issue9pt_PT
oaire.citation.startPage2292pt_PT
oaire.citation.titleGenome Biology and Evolutionpt_PT
oaire.citation.volume9pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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