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Exploring the contribution of mycobacteria characteristics in the interaction with human macrophages

2013-06-24Journal article Restricted access
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dc.contributor.authorSilva, Carla
dc.contributor.authorPerdigao, Joao
dc.contributor.authorAlverca, Elsa
dc.contributor.authorde Matos, António Pedro Alves
dc.contributor.authorCarvalho, Patrícia A
dc.contributor.authorPortugal, Isabel
dc.contributor.authorJordão, Luísa
dc.date.accessioned2013-07-29T16:14:27Z
dc.date.available2013-07-29T16:14:27Z
dc.date.issued2013-06-24
dc.description.abstractTuberculosis (TB) is a major health problem. The emergence of multidrug resistant (MDR) Mycobacterium tuberculosis (Mtb) isolates confounds treatment strategies. In Portugal, cases of MDR-TB are reported annually with increased incidence noted in Lisbon. The majority of these MDR-TB cases are due to closely-related mycobacteria known collectively as Lisboa family and Q1 cluster. The genetic determinants linked to drug resistance have been exhaustively studied resulting in the identification of family and cluster specific mutations. Nevertheless little is known about other factors involved in drug resistance development. Here, for the first time, we complemented the genetic analysis with the study of morphological and structural features of Lisboa family and Q1 cluster isolates by using scanning and transmission electron microscopy. This analysis allowed the identification of structural differences, such as cell envelope thickness, between Mtb clinical isolates, which are correlated with antibiotic resistance. The infection of human monocyte derived macrophages allowed us to document the relative selective advantage of Lisboa family isolates over other circulating Mtb isolates.por
dc.identifier.citationMicrosc Microanal. 2013 Oct;19(5):1159-69. doi: 10.1017/S1431927613001906. Epub 2013 Jun 24.por
dc.identifier.issn1431-9276
dc.identifier.otherdoi:10.1017/S1431927613001906
dc.identifier.urihttp://hdl.handle.net/10400.18/1696
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherCambridge University Press / Microscopy Society of Americapor
dc.relation.publisherversionhttp://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=8943875por
dc.subjectMycobacteriumpor
dc.subjectTuberculosispor
dc.subjectHost-pathogen Interactionpor
dc.subjectDrug-resistancepor
dc.subjectTEMpor
dc.subjectSEMpor
dc.subjectMIRUSpor
dc.subjectCell Envelopepor
dc.subjectInfecções Respiratóriaspor
dc.titleExploring the contribution of mycobacteria characteristics in the interaction with human macrophagespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceCambridge, UKpor
oaire.citation.endPage11por
oaire.citation.startPage1por
oaire.citation.titleMicroscopy and Microanalysispor
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

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