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Simultaneous speciation analysis of Hg and Se in fish by high-performance liquid chromatography and inductively coupled plasma-mass spectrometry following microwave-assisted enzymatic hydrolysis

2023-10-11Journal article Restricted access
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dc.contributor.authorRibeiro, M.
dc.contributor.authorGalli, E.
dc.contributor.authorGuérin, T.
dc.contributor.authorSilva, J.A.L.
dc.contributor.authorCastanheira, I.
dc.contributor.authorLeufroy, A.
dc.contributor.authorJitaru, P.
dc.date.accessioned2024-01-24T13:54:15Z
dc.date.available2024-01-24T13:54:15Z
dc.date.issued2023-10-11
dc.description.abstractThis study reports the development and validation of a new analytical method for simultaneous speciation analysis of Se and Hg in fish muscle. For this purpose, four Se species (selenite/Se(IV), selenate/Se(VI), selenomethionine/SeMet, and selenocysteine/SeCys) and two Hg species (inorganic mercury/iHg and methylmercury/MeHg) were extracted simultaneously by microwave-assisted enzymatic hydrolysis and then separated by HPLC in less than 15 min by using a column with both anion and cation exchange mechanisms and a mobile phase consisting of a mixture of methanol 5% (v/v), 45 mM HNO3, 0.015% 2-mercaptoethanol, and 1.5 mM sodium 3-mercapto-1-propanesulfonate. The separated species of Hg and Se were detected online by inductively coupled plasma-mass spectrometry (ICP-MS). The speciation analysis method was validated by means of the accuracy profile approach by carrying out three series of measurements in duplicate on three different days over a time-span of 3 weeks. The limits of quantification (LOQ) are in the range of 0.010–0.013 mg/kg wet weight (ww) for all selenium species, except for Se(IV) (0.15 mg/kg ww), while the coefficient of variation in terms of intermediate reproducibility (CVR) was < 7%. The LOQ for MeHg was 0.006 mg/kg ww, while the CVR was 3%. The method was successfully applied to the analysis of muscle samples from four different fish species: rainbow trout, tuna, swordfish, and dogfish.pt_PT
dc.description.sponsorshipThis study is a contribution to the Ph.D. research project (MERSEL-FISH) and it was jointly funded by ANSES (France) and INSA (Portugal).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAnal Bioanal Chem. 2023 Dec;415(29-30):7175-7186. doi: 10.1007/s00216-023-04984-1. Epub 2023 Oct 11.pt_PT
dc.identifier.doi10.1007/s00216-023-04984-1pt_PT
dc.identifier.issn1618-2642
dc.identifier.urihttp://hdl.handle.net/10400.18/8962
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s00216-023-04984-1pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHg Speciationpt_PT
dc.subjectEnzymatic Hydrolysispt_PT
dc.subjectHPLC-ICP-MSpt_PT
dc.subjectAccuracy Profilept_PT
dc.subjectFishpt_PT
dc.subjectSe Speciationpt_PT
dc.subjectComposição dos Alimentospt_PT
dc.titleSimultaneous speciation analysis of Hg and Se in fish by high-performance liquid chromatography and inductively coupled plasma-mass spectrometry following microwave-assisted enzymatic hydrolysispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage7186pt_PT
oaire.citation.issue29-30pt_PT
oaire.citation.startPage7175pt_PT
oaire.citation.titleAnalytical and Bioanalytical Chemistrypt_PT
oaire.citation.volume415pt_PT
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT

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