Repository logo
 
Publication

Coinfections of Rickettsia slovaca and Rickettsia helvetica with Borrelia lusitaniae in ticks collected in a Safari Park, Portugal

2010-12Journal article Restricted access
Simple item page
dc.contributor.authorMilhano, Natacha
dc.contributor.authorCarvalho, Isabel Lopes de
dc.contributor.authorAlves, Ana Sofia
dc.contributor.authorArroube, Sofia
dc.contributor.authorSoares, Jorge
dc.contributor.authorRodriguez, Pablo
dc.contributor.authorCarolino, Manuela
dc.contributor.authorNúncio, Maria Sofia
dc.contributor.authorPiesman, Joseph
dc.contributor.authorSousa, Rita de
dc.date.accessioned2011-09-17T08:26:21Z
dc.date.available2011-09-17T08:26:21Z
dc.date.issued2010-12
dc.description.abstractBorrelia and Rickettsia bacteria are the most important tick-borne agents causing disease in Portugal. Identification and characterization of these circulating agents, mainly in recreational areas, is crucial for the development of preventive measures in response to the gradually increasing exposure of humans to tick vectors. A total of 677 questing ticks including Dermacentor marginatus, Rhipicephalus sanguineus, Ixodes ricinus, Hyalomma lusitanicum, H. marginatum, and Haemaphysalis punctata were collected in a Safari Park in Alentejo, Portugal, to investigate the prevalences of infection and characterize Borrelia and Rickettsia species. From a total of 371 ticks tested by PCR for Borrelia burgdorferi sensu lato (s.l.), of which 247 were tested for Rickettsia, an infection prevalence of 18.3% was found for B. lusitaniae and 55.1% for Rickettsia spp. Sequence analysis of positive amplicons identified the presence of B. lusitaniae (18.3%), R. monacensis strain IRS3 (51.7%), and R. helvetica (48.3%) in I. ricinus. R. slovaca (41.5%), R. raoultii (58.5%), and also B. lusitaniae (21%) were identified in D. marginatus ticks. One (5.9%) H. lusitanicum was infected with B. lusitaniae, and R. massiliae was found in one Rhipicephalus sanguineus. Coinfection was found in 7 (20%) I. ricinus and 34 (23.3%) D. marginatus ticks. We report, for the first time, simultaneous infection with R. helvetica and B. lusitaniae and also R. slovaca, the agent of TIBOLA/DEBONEL, with B. lusitaniae. Additionally, 6 isolates of B. lusitaniae were established, and isolates of Rickettsia were also obtained for the detected species using tick macerates cultured in mammalian and mosquito cell lines. This report describes the detection and isolation of tick-borne agents from a Portuguese Safari Park, highlighting the increased likelihood of infection with multiple agents to potential visitors or staff.por
dc.identifier.citationTicks Tick Borne Dis. 2010 Dec;1(4):172-7por
dc.identifier.issn1877-959X
dc.identifier.otherdoi:10.1016/j.ttbdis.2010.09.003
dc.identifier.urihttp://hdl.handle.net/10400.18/185
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S1877959X10000713por
dc.subjectTickspor
dc.subjectRickettsiapor
dc.subjectBorreliapor
dc.subjectSafari parkpor
dc.subjectPortugalpor
dc.subjectEstudos de Vectores e Doenças Infecciosaspor
dc.titleCoinfections of Rickettsia slovaca and Rickettsia helvetica with Borrelia lusitaniae in ticks collected in a Safari Park, Portugalpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage177por
oaire.citation.startPage172por
oaire.citation.titleTicks and Tick-borne Diseasespor
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Coinfections of Rickettsia slovaca and Rickettsia.pdf
Size:
202.13 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

DDI - Artigos em revistas internacionais