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Helicobacter pullorum Isolated from Fresh Chicken Meat: Antibiotic Resistance and Genomic Traits of an Emerging Foodborne Pathogen

2015-12Journal article Restricted access
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dc.contributor.authorBorges, Vítor
dc.contributor.authorSantos, Andrea
dc.contributor.authorCorreia, Cristina Belo
dc.contributor.authorSaraiva, Margarida
dc.contributor.authorMénard, Armelle
dc.contributor.authorVieira, Luís
dc.contributor.authorSampaio, Daniel A.
dc.contributor.authorPinheiro, Miguel
dc.contributor.authorGomes, João Paulo
dc.contributor.authorOleastro, Mónica
dc.date.accessioned2016-02-18T17:55:37Z
dc.date.available2016-12-01T01:30:08Z
dc.date.issued2015-12
dc.description.abstractMeat and meat products are important sources of human intestinal infections. We report the isolation of Helicobacter pullorum strains from chicken meat. Bacteria were isolated from 4 of the 17 analyzed fresh chicken meat samples, using a membrane filter method. MIC determination revealed that the four strains showed acquired resistance to ciprofloxacin; one was also resistant to erythromycin, and another one was resistant to tetracycline. Whole-genome sequencing of the four strains and comparative genomics revealed important genetic traits within the H. pullorum species, such as 18 highly polymorphic genes (including a putative new cytotoxin gene), plasmids, prophages, and a complete type VI secretion system (T6SS). The T6SS was found in three out of the four isolates, suggesting that it may play a role in H. pullorum pathogenicity and diversity. This study suggests that the emerging pathogen H. pullorum can be transmitted to humans by chicken meat consumption/contact and constitutes an important contribution toward a better knowledge of the genetic diversity within the H. pullorum species. In addition, some genetic traits found in the four strains provide relevant clues to how this species may promote adaptation and virulence.pt_PT
dc.description.sponsorshipThis work was funded by the Portuguese National Institute of Health grant for Research and Development.pt_PT
dc.identifier.citationAppl Environ Microbiol. 2015 Dec;81(23):8155-63. doi: 10.1128/AEM.02394-15. Epub 2015 Sep 18pt_PT
dc.identifier.doi10.1128/AEM.02394-15pt_PT
dc.identifier.issn0099-2240
dc.identifier.urihttp://hdl.handle.net/10400.18/3433
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Society for Microbiologypt_PT
dc.relation.publisherversionhttp://aem.asm.org/content/81/23/8155.abstractpt_PT
dc.subjectHelicobacter pullorumpt_PT
dc.subjectEmergent Foodborne Pathogenpt_PT
dc.subjectVirulencept_PT
dc.subjectAntibiotic Resistancept_PT
dc.subjectChicken Meatpt_PT
dc.subjectWhole Genome Sequencingpt_PT
dc.subjectInfecções Gastrointestinaispt_PT
dc.titleHelicobacter pullorum Isolated from Fresh Chicken Meat: Antibiotic Resistance and Genomic Traits of an Emerging Foodborne Pathogenpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage8163pt_PT
oaire.citation.startPage8155pt_PT
oaire.citation.titleApplied and Environmental Microbiologypt_PT
oaire.citation.volume81(23)pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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