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Genomic Characterization of Multidrug-Resistant and ESBL-Producing Klebsiella pneumoniae Isolated from Healthy Rabbits Intended for Human Consumption

2025-08-18Artigo científico Acesso aberto
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dc.contributor.authorSilva, Vanessa
dc.contributor.authorCaniça, Manuela
dc.contributor.authorRivière, Rani
dc.contributor.authorSilva, Adriana
dc.contributor.authorPoeta, Patrícia
dc.contributor.authorIgrejas, Gilberto
dc.date.accessioned2026-02-05T11:19:38Z
dc.date.available2026-02-05T11:19:38Z
dc.date.issued2025-08-18
dc.description(This article belongs to the Special Issue Novel Sequencing Technologies for the Characterization of Multidrug-Resistant Bacteria)
dc.description.abstractKlebsiella pneumoniae is an important pathogen associated with multidrug resistance and virulence in both human and animal populations. While its prevalence and resistance patterns are well documented in clinical settings, data on K. pneumoniae in food-producing animals remain scarce. This study aimed to isolate and characterize multidrug-resistant K. pneumoniae strains from healthy rabbits raised for human consumption, with a focus on antimicrobial resistance genes, plasmid content, and associated mobile genetic elements. A total of 295 fecal samples were collected from rabbits across 20 commercial farms in northern Portugal. Isolates were confirmed using MALDI-TOF MS, tested for hypermucoviscosity, and subjected to antimicrobial susceptibility testing (EUCAST). Whole-genome sequencing (WGS) was performed to determine sequence types (STs), resistance genes, plasmids, and resistance determinants for metals and biocides. Six K. pneumoniae isolates were recovered, showing extensive antimicrobial resistance profiles, including ESBL genes such as blaCTX-M-15, blaSHV-28, and blaTEM-1. The most frequent ST was ST307. Multiple genes resistant to heavy metals were identified. Plasmid analysis revealed the presence of IncFII, IncN, and ColRNAI types. Network analysis showed clusters of genetically related isolates and highlighted shared resistance mechanisms. The presence of multidrug-resistant K. pneumoniae in healthy rabbits destined for human consumption underscores the zoonotic potential of this species and the need for surveillance in the animal-food-human interface. These findings contribute to a better understanding of resistance ecology in the context of One Health.eng
dc.description.sponsorshipThis work was supported by the projects UI/00772 and LA/P/0059/2020, funded by the Portuguese Foundation for Science and Technology (FCT). This work received financial support from FCT/MCTES (UIDB/00772/2020, DOI: 10.54499/UIDB/00772/2020; UIDB/50006/2020, DOI: 10.54499/UIDB/50006/2020; LA/P/0008/2020, DOI: 10.54499/LA/P/0008/2020; UIDP/50006/2020, DOI: 10.54499/UIDP/50006/2020.
dc.identifier.citationMicroorganisms.2025 Aug 18;13(8):1931. doi: 10.3390/microorganisms13081931
dc.identifier.doi10.3390/microorganisms13081931
dc.identifier.eissn2076-2607
dc.identifier.pmid40871435
dc.identifier.urihttp://hdl.handle.net/10400.18/10812
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationAssociate Laboratory for Animal and Veterinary Sciences
dc.relationVeterinary and Animal Science Research Centre
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relation.hasversionhttps://www.mdpi.com/2076-2607/13/8/1931
dc.relation.ispartofseries1
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectESBL
dc.subjectKlebsiella pneumoniae
dc.subjectOne Health
dc.subjectAntimicrobial Resistance
dc.subjectRabbits
dc.subjectWhole-Genome Sequencing
dc.subjectResistência aos Antimicrobianos
dc.titleGenomic Characterization of Multidrug-Resistant and ESBL-Producing Klebsiella pneumoniae Isolated from Healthy Rabbits Intended for Human Consumptioneng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAssociate Laboratory for Animal and Veterinary Sciences
oaire.awardTitleVeterinary and Animal Science Research Centre
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0059%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00772%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PT
oaire.citation.issue8
oaire.citation.startPage1931
oaire.citation.titleMicroorganisms
oaire.citation.volume13
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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