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The influence of short-chain fatty acids on the survival and virulence of Arcobacter butzleri

2025-11-28Artigo científico Acesso restrito
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dc.contributor.authorFonseca, Inês M.
dc.contributor.authorMateus, Cristiana
dc.contributor.authorVieira, Alexandre
dc.contributor.authorDomingues, Fernanda
dc.contributor.authorManageiro, Vera
dc.contributor.authorOleastro, Mónica
dc.contributor.authorFerreira, Susana
dc.date.accessioned2026-01-22T10:41:17Z
dc.date.available2026-01-22T10:41:17Z
dc.date.issued2025-11-28
dc.description.abstractAims: Arcobacter butzleri, a widespread bacterium linked to gastrointestinal disease, can bypass host colonization resistance mechanisms; however, its response to short-chain fatty acids (SCFAs) remains poorly understood. This study investigated the impact of SCFAs on A. butzleri ’s survival and virulence. Methods and results: Eight A. butzleri isolates were assessed under varying concentrations of individual SCFAs and mixtures (m-SCFAs). Higher SCFAs concentrations inhibited bacterial growth in a strain-dependent manner. Transcript analysis of putative virulence genes revealed upregula- tion of ciaB and flaA across most m-SCFAs concentrations, while luxS expression increased at 90 mM. SCFAs generally reduced bacterial motility, with sodium propionate reducing motility but enhancing biofilm-forming ability in the model strain. Additionally, SCFAs exposure decreased the ability of A. butzleri to adhere to and invade the Caco-2 intestinal epithelial cell line. Whole-genome sequencing of the eight A. butzleri isolates revealed extensive genetic diversity, particularly in virulence- and stress-associated genes, although consistent genot ype/phenot ype correlations were not observed. Conclusions: Altogether, these findings demonstrate that SCFAs modulate A. butzleri survival and virulence, providing novel insights into their significance in shaping pathogen behaviour and host-pathogen interactions.eng
dc.description.sponsorshipCristiana Mateus is recipient of a doctoral fellowship (UI/BD/151023/2021) under the scope of the CICS-UBI Programmatic Funding (UIDP/00709/2020). This work was developed within the scope of the CICS-UBI projects UIDB/00709/2020 and UIDP/00709/2020, financed by national funds through the Portuguese Foundation for Science and Technology/MCTES.
dc.identifier.citationJ Appl Microbiol. 2025 Nov 28;136(12):lxaf303. doi: 10.1093/jambio/lxaf303
dc.identifier.doi10.1093/jambio/lxaf303
dc.identifier.eissn1365-2672
dc.identifier.issn1364-5072
dc.identifier.pmid41384789
dc.identifier.urihttp://hdl.handle.net/10400.18/10736
dc.language.isoeng
dc.peerreviewedyes
dc.publisherOxford University Press
dc.relationUnravelling the virulence potential of Aliarcobacter butzleri
dc.relationHealth Sciences Research Centre
dc.relationHealth Sciences Research Centre
dc.relation.hasversionhttps://academic.oup.com/jambio/article-abstract/136/12/lxaf303/8378085?redirectedFrom=fulltext
dc.relation.ispartofJournal of Applied Microbiology
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectArcobacter Butzleri
dc.subjectAdhesion and Invasion
dc.subjectBiofilms
dc.subjectMotility
dc.subjectShort-Chain Fatty Acids
dc.subjectVirulence Genes
dc.subjectResistência aos Antimicrobianos
dc.subjectInfecções Gastrointestinais
dc.titleThe influence of short-chain fatty acids on the survival and virulence of Arcobacter butzlerieng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleUnravelling the virulence potential of Aliarcobacter butzleri
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleHealth Sciences Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//UI%2FBD%2F151023%2F2021/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.citation.issue12
oaire.citation.startPagelxaf303
oaire.citation.titleJournal of Applied Microbiology
oaire.citation.volume136
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isProjectOfPublication60f6a70b-110d-4c15-a3fd-d43d078078bc
relation.isProjectOfPublicatione0fbaa7c-391a-479d-9bcf-efb7997d4004
relation.isProjectOfPublicationced21566-cfde-4640-afff-0536a07c1f8f
relation.isProjectOfPublication.latestForDiscovery60f6a70b-110d-4c15-a3fd-d43d078078bc

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