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SELDI-TOF biomarker signatures for cystic fibrosis, asthma and chronic obstructive pulmonary disease

2010-01Journal article Restricted access
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dc.contributor.authorGomes-Alves, Patrícia
dc.contributor.authorImrie, Margaret
dc.contributor.authorGray, Robert D.
dc.contributor.authorNogueira, Paulo
dc.contributor.authorCiordia, Sergio
dc.contributor.authorPacheco, Paula
dc.contributor.authorAzevedo, Pilar
dc.contributor.authorLopes, Carlos
dc.contributor.authorDe Almeida, António Bugalho
dc.contributor.authorGuardiano, Micaela
dc.contributor.authorPorteous, David J.
dc.contributor.authorAlbard, Juan P.
dc.contributor.authorBoyd, A. Christopher
dc.contributor.authorPenque, Deborah
dc.date.accessioned2011-09-13T16:45:46Z
dc.date.available2011-09-13T16:45:46Z
dc.date.issued2010-01
dc.description.abstractOBJECTIVES: The aim of this work was to establish protein profiles in serum and nasal epithelial cells of cystic fibrosis individuals in comparison with controls, asthma and chronic obstructive pulmonary disease patients for specific biomarker signatures identification. DESIGN AND METHODS: Protein extracts were analyzed by Surface Enhanced Laser Desorption/Ionization Time-Of-Flight Mass-Spectrometry (SELDI-TOF-MS). RESULTS: The mass spectra revealed a set of peaks with differential expression in serum and nasal cells among the different groups studied, resulting into peak signatures representative/specific of each pathology. Logistic regressions were applied to those peaks; sensitivity, specificity, Youden's indexes and area under the curve (AUC) of the respective receiver operating characteristic (ROC) curves were compared. DISCUSSION: Multivariate analysis demonstrated that combination of peaks has a better predictive value than the individual ones. These protein signatures may serve as diagnostic/prognostic markers for the studied diseases with common clinical features, or as follow-up assessment markers of therapeutic interventions.por
dc.identifier.citationClin Biochem. 2010 Jan;43(1-2):168-77. Epub 2009 Oct 20por
dc.identifier.issn0009-9120
dc.identifier.otherdoi:10.1016/j.clinbiochem.2009.10.006
dc.identifier.urihttp://hdl.handle.net/10400.18/159
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0009912009004469por
dc.subjectCFpor
dc.subjectAsthmapor
dc.subjectCOPDpor
dc.subjectProteomicspor
dc.subjectSELDI-TOF-MSpor
dc.subjectBiomarkers signaturespor
dc.subjectDoenças Genéticaspor
dc.subjectGenómica Funcional e Estruturalpor
dc.subjectDeterminantes da Saúde e da Doençapor
dc.titleSELDI-TOF biomarker signatures for cystic fibrosis, asthma and chronic obstructive pulmonary diseasepor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage177por
oaire.citation.startPage168por
oaire.citation.titleClinical Biochemistrypor
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

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