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Variation of DNA damage levels in peripheral blood mononuclear cells isolated in different laboratories

2014-07Journal article Restricted access
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dc.contributor.authorGodschalk, R.W.
dc.contributor.authorErsson, C.
dc.contributor.authorStępnik, M.
dc.contributor.authorFerlińska, M.
dc.contributor.authorPalus, J.
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorCosta, S.
dc.contributor.authorJones, G.D.
dc.contributor.authorHiggins, J.A.
dc.contributor.authorKain, J.
dc.contributor.authorMöller, L.
dc.contributor.authorForchhammer, L.
dc.contributor.authorLoft, S.
dc.contributor.authorLorenzo, Y.
dc.contributor.authorCollins, A.R.
dc.contributor.authorvan Schooten, F.J.
dc.contributor.authorLaffon, B.
dc.contributor.authorValdiglesias, V.
dc.contributor.authorCooke, M.
dc.contributor.authorMistry, V.
dc.contributor.authorKarbaschi, M.
dc.contributor.authorPhillips, D.H.
dc.contributor.authorSozeri, O.
dc.contributor.authorRoutledge, M.N.
dc.contributor.authorNelson-Smith, K.
dc.contributor.authorRiso, P.
dc.contributor.authorPorrini, M.
dc.contributor.authorLópez de Cerain, A.
dc.contributor.authorAzqueta, A.
dc.contributor.authorMatullo, G.
dc.contributor.authorAllione, A.
dc.contributor.authorMøller, P.
dc.date.accessioned2015-02-03T13:10:07Z
dc.date.available2015-02-03T13:10:07Z
dc.date.issued2014-07
dc.description.abstractThis study investigated the levels of DNA strand breaks and formamidopyrimidine DNA glycosylase (FPG) sensitive sites, as assessed by the comet assay, in peripheral blood mononuclear cells (PBMC) from healthy women from five different countries in Europe. The laboratory in each country (referred to as 'centre') collected and cryopreserved PBMC samples from three donors, using a standardised cell isolation protocol. The samples were analysed in 13 different laboratories for DNA damage, which is measured by the comet assay. The study aim was to assess variation in DNA damage in PBMC samples that were collected in the same way and processed using the same blood isolation procedure. The inter-laboratory variation was the prominent contributor to the overall variation. The inter-laboratory coefficient of variation decreased for both DNA strand breaks (from 68 to 26%) and FPG sensitive sites (from 57 to 12%) by standardisation of the primary comet assay endpoint with calibration curve samples. The level of DNA strand breaks in the samples from two of the centres (0.56-0.61 lesions/10(6) bp) was significantly higher compared with the other three centres (0.41-0.45 lesions/10(6) bp). In contrast, there was no difference between the levels of FPG sensitive sites in PBMC samples from healthy donors in the different centres (0.41-0.52 lesion/10(6) bp).por
dc.description.sponsorshipEnvironmental Cancer Risk, Nutrition and Individual Susceptibility 2 (ECNIS2) towards the establishment of a virtual European Centre for Research and Education on Cancer, Environment and Food, European Commission FP7-KBBE-2010–4 (Contract No. 266198).por
dc.identifier.citationMutagenesis. 2014 Jul;29(4):241-9. doi: 10.1093/mutage/geu012. Epub 2014 Apr 15.por
dc.identifier.doi10.1093/mutage/geu012
dc.identifier.issn0267-8357
dc.identifier.urihttp://hdl.handle.net/10400.18/2752
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherOxford University Press/UK Environmental Mutagen Societypor
dc.relation.publisherversionhttp://mutage.oxfordjournals.org/content/29/4/241.abstractpor
dc.subjectDNA Damagepor
dc.subjectComet Assaypor
dc.subjectEnvironmental Cancer Riskpor
dc.subjectAr e Saúde Ocupacionalpor
dc.subjectGenotoxicidade Ambientalpor
dc.titleVariation of DNA damage levels in peripheral blood mononuclear cells isolated in different laboratoriespor
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/266198
oaire.citation.endPage249por
oaire.citation.startPage241por
oaire.citation.titleMutagenesispor
oaire.citation.volume29(4)por
oaire.fundingStreamFP7
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsembargoedAccesspor
rcaap.typearticlepor
relation.isProjectOfPublication7a7427c2-d7eb-4009-ac82-537cc067c18e
relation.isProjectOfPublication.latestForDiscovery7a7427c2-d7eb-4009-ac82-537cc067c18e

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