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Self-assembled dextrin nanogel as protein carrier: Controlled release and biological activity of IL-10

2011-03-09Journal article Restricted access
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dc.contributor.authorCarvalho, V.
dc.contributor.authorCastanheira, P.
dc.contributor.authorMadureira, P.
dc.contributor.authorFerreira, S.
dc.contributor.authorCosta, C.
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorFaro, C.
dc.contributor.authorVilanova, M.
dc.contributor.authorGama, M.
dc.date.accessioned2012-01-31T12:14:05Z
dc.date.available2012-01-31T12:14:05Z
dc.date.issued2011-03-09
dc.description.abstractInterleukin-10 (IL-10) is an anti-inflammatory cytokine, which active form is a non-covalent homodimer. Given the potential of IL-10 for application in various medical conditions, it is essential to develop systems for its effective delivery. In previous work, it has been shown that a dextrin nanogel effectively incorporated and stabilized rIL-10, enabling its release over time. In this work, the delivery system based on dextrin nanogels was further analyzed. The biocompatibility of the nanogel was comprehensively analyzed, through cytotoxicity (lactate dehydrogenase (LDH) release, MTS, Live, and Dead) and genotoxicity (comet) assays. The release profile of rIL-10 and its biological activity were evaluated in vivo, using C57BL/6 mice. Although able to maintain a stable concentration of IL-10 for at least 4 h in mice serum, the amount of protein released was rather low. Despite this, the amount of rIL-10 released from the complex was biologically active inhibiting TNF-α production, in vivo, by LPS-challenged mice. In spite of the significant stabilization achieved using the nanogel, rIL-10 still denatures rather quickly. An additional effort is thus necessary to develop an effective delivery system for this cytokine, able to release active protein over longer periods of time. Nevertheless, the good biocompatibility, the protein stabilization effect and the ability to perform as a carrier with controlled release suggest that self-assembled dextrin nanogels may be useful protein delivery systems.por
dc.description.sponsorshipFCT grant SFRH/BD/27359/2006.This study was financially supported by FCT Project PTDC/BIO/67160/2006 and grant SUDOE-FEDERIMMUNONETSOE1/P1/E014por
dc.identifier.citationBiotechnol Bioeng. 2011 Aug;108(8):1977-86. Epub 2011 Mar 22por
dc.identifier.issn0006-3592
dc.identifier.otherdoi: 10.1002/bit.23125
dc.identifier.urihttp://hdl.handle.net/10400.18/452
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWiley-Blackwellpor
dc.relationFCT Project PTDC/BIO/67160/2006 and grant SUDOE-FEDERIMMUNONETSOE1/P1/E014por
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1002/bit.23125/abstract;jsessionid=537E37ECB0E2A5D4956D8B8F09F21BC9.d01t02?systemMessage=Wiley+are+currently+testing+a+Chinese+version+of+Wiley+Online+Library.+This+functionality+will+be+turned+on+for+a+short+period+of+time+only.+Apologies+for+any+inconvenience..por
dc.subjectProtein Delivery Systempor
dc.subjectDextrin Nanogelpor
dc.subjectBiocompatibilitypor
dc.subjectInterleukin-10por
dc.subjectAnti-inflammatory Agentpor
dc.titleSelf-assembled dextrin nanogel as protein carrier: Controlled release and biological activity of IL-10por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1986por
oaire.citation.startPage1977por
oaire.citation.titleBiotechnology and Bioengineeringpor
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

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