Repository logo
 
Publication

Toxicity screening of a novel poly(methylmethacrylate)-Eudragit nanocarrier on L929 fibroblasts

2017-07-05Journal article Restricted access
Simple item page
dc.contributor.authorGraça, Diogo
dc.contributor.authorLouro, Henriqueta
dc.contributor.authorSantos, Joana
dc.contributor.authorDias, Kamila
dc.contributor.authorAlmeida, Antonio J
dc.contributor.authorGonçalves, Lídia
dc.contributor.authorSilva, Maria João
dc.contributor.authorBettencourt, Ana
dc.date.accessioned2017-07-06T11:05:35Z
dc.date.available2021-07-06T00:30:09Z
dc.date.issued2017-07-05
dc.description.abstractTranslation of innovative drug delivery nanosystems into the market involves an early toxicity screening in the development phase. Previously, we showed that inclusion of the polymer Eudragit (EUD) into poly(methylmethacrylate) (PMMA) nanoparticles (NP) resulted in a novel nanocarrier (PMMA-EUD) with an improved biomedical performance. The safety of this novel nanoparticulate system (PMMA-EUDNPs) was assessed in this work and compared to that of the original PMMANPs by using an integrated approach, comprising in vitro toxicity assessment and NPs physicochemical characterization in water and cell medium. For toxicity assessment several endpoints were analysed, including cell death, oxidative stress, and genotoxicity using L929 fibroblasts. PMMA-EUDNPs proved to be more hydrophobic than the original PMMANPs. Also, charge of both NPs was strongly affected by cell medium. On the other hand, the novel nanosystem was easily uptaken by L929 cells and did not display relevant in vitro cytotoxic or genotoxic effects. On the contrary, PMMANPs were less internalized in cells and proved to be genotoxic, as measured by the micronucleus assay. To conclude, our results provide initial evidence about the safety of the novel and promising PMMA-EUD nanoparticulate system, enabling its further development towards applications for drug delivery.pt_PT
dc.description.sponsorshipFundação para a Ciência e a Tecnologia (projects: EXCL/CTM-NAN/0166/2012, Pest-UID/DTP/04138/2013, ToxOmics, UID/BIM/00009/2013)pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationToxicol Lett. 2017 Jul 5;276:129-137. doi: 10.1016/j.toxlet.2017.05.017. Epub 2017 May 17pt_PT
dc.identifier.doi10.1016/j.toxlet.2017.05.017pt_PT
dc.identifier.issn0378-4274
dc.identifier.urihttp://hdl.handle.net/10400.18/4746
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier/EUROTOXpt_PT
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0378427417301959pt_PT
dc.subjectNanoparticles Characterizationpt_PT
dc.subjectNanomedicinept_PT
dc.subjectNanotoxicologypt_PT
dc.subjectPolymeric Nanocarrierspt_PT
dc.subjectGenotoxicitypt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleToxicity screening of a novel poly(methylmethacrylate)-Eudragit nanocarrier on L929 fibroblastspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/EXCL%2FCTM-NAN%2F0166%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FDTP%2F04138%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIM%2F00009%2F2013/PT
oaire.citation.endPage137pt_PT
oaire.citation.startPage129pt_PT
oaire.citation.titleToxicology Letterspt_PT
oaire.citation.volume276pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication3b03b44b-4527-44bd-a501-a255c86d92f1
relation.isProjectOfPublication8b7dc2e1-c04d-4a11-8fa9-83ead9d8f256
relation.isProjectOfPublicatione9cc9728-4f09-4e3a-b30d-53d4429986fb
relation.isProjectOfPublication.latestForDiscovery3b03b44b-4527-44bd-a501-a255c86d92f1

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
2017_Graça2017.pdf
Size:
1 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

DGH - Artigos em revistas internacionais