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Influence of dietary patterns on contaminants bioaccessibility and intestinal transport by in vitro assays

2020-11Journal article Restricted access
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dc.contributor.authorFaria, M.A.
dc.contributor.authorMelo, A.
dc.contributor.authorFerreira, Isabel I.M.P.L.V.O.
dc.date.accessioned2021-04-08T10:08:25Z
dc.date.available2021-04-08T10:08:25Z
dc.date.issued2020-11
dc.description.abstractFood contaminants are usually studied as isolated molecules, not considering the overall meal components. Notwithstanding, contaminants are not ingested individually, therefore their risks should be assessed in the context of the overall diet. In the present study the influence of three well known dietary patterns, Western (W), Mediterranean (M) and vegetarian (V), on the bioaccessibility and intestinal transport of polycyclic aromatic hydrocarbons (PAHs) (benzo[a]pyrene (BaP) and benzo[b]fluoranthene (BbF)), heterocyclic aromatic amines (HAAs) (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) and 2-Amino-9H-pyrido[2,3-b]indole (AAC)) and mycotoxins (MY) (aflatoxin B2 (AB2) and ochratoxin A (OTA)) was evaluated. Whole meals representative of W, M and V patterns were spiked with 100 µg kg-1 of each contaminant and subjected to the Infogest in vitro digestion method. Intestinal transport was performed using Caco-2 cells in apical/basolateral inserts. Contaminants were quantified by QuEChERS/HPLC/Fluorescence analysis. The dietary pattern itself influenced significantly the bioaccessibility of some contaminants, since higher bioaccessibility of HAAs (PhIP and AAC) was observed for V diet, while higher bioaccessibility of PAHs (BBF and BAP) and the MY (OTA) was observed for W diet. Concerning intestinal transport, the effect of the diet matrices was less noticed. Notwithstanding, AAC transport increased with W diet, while AB2s transport increased with the V diet. Regarding PAHs the three patterns either blocked (BbF) or reduced (BaP) the transport. Besides the well known nutritional, protective or deleterious effects of the different dietary patterns, the increased bioaccessibility or intestinal transport of some food contaminants, can have an additional influence on the global health impact.pt_PT
dc.description.abstractHighlights: Dietary pattern influenced the bioaccessibility of some studied food contaminants; The impact of dietary pattern on intestinal transport was less notorious; Dietary pattern affects contaminants bioaccessibility/absorption and health.pt_PT
dc.description.sponsorshipM. A. Faria acknowledges Fundação para a Ciência e a Tecnologia and Norte-01-0145-FEDER-0001 the researcher contract. This work was financed by FEDER - Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020 - Operational Programme for Competitiveness and Internationalisation (POCI), and by Portuguese funds through FCT - Fundação para a Ciência e a Tecnologia in the framework of the project PTDC/SAU-NUT/30322/2017.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFood Res Int. 2020 Nov;137:109358. doi: 10.1016/j.foodres.2020.109358. Epub 2020 May 28.pt_PT
dc.identifier.doi10.1016/j.foodres.2020.109358pt_PT
dc.identifier.issn0963-9969
dc.identifier.urihttp://hdl.handle.net/10400.18/7665
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier/ Canadian Institute of Food Science and Technologypt_PT
dc.relationDiet impac on the prevention of chronic diseases: a foodomics approach
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/abs/pii/S0963996920303835?via%3Dihubpt_PT
dc.subjectCaco-2 Cellspt_PT
dc.subjectHeterocyclic Aminespt_PT
dc.subjectINFOGEST Digestion Methodpt_PT
dc.subjectIntestinal Transport; Mediterranean Dietpt_PT
dc.subjectMycotoxinspt_PT
dc.subjectPolycyclic Aromatic Hydrocarbonspt_PT
dc.subjectVegetarian Dietpt_PT
dc.subjectWestern Dietpt_PT
dc.subjectÁgua e Solopt_PT
dc.titleInfluence of dietary patterns on contaminants bioaccessibility and intestinal transport by in vitro assayspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDiet impac on the prevention of chronic diseases: a foodomics approach
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FSAU-NUT%2F30322%2F2017/PT
oaire.citation.startPage109358pt_PT
oaire.citation.titleFood Research Internationalpt_PT
oaire.citation.volume137pt_PT
oaire.fundingStream9471 - RIDTI
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication7a4cc6c5-dc89-4f3d-9f2e-b2a27dd36cd5
relation.isProjectOfPublication.latestForDiscovery7a4cc6c5-dc89-4f3d-9f2e-b2a27dd36cd5

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