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Fucoxanthin Holds Potential to Become a Drug Adjuvant in Breast Cancer Treatment: Evidence from 2D and 3D Cell Cultures

2021-07-15Journal article Open access
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dc.contributor.authorMalhão, Fernanda
dc.contributor.authorMacedo, Ana Catarina
dc.contributor.authorCosta, Carla
dc.contributor.authorRocha, Eduardo
dc.contributor.authorRamos, Alice Abreu
dc.date.accessioned2022-07-06T15:56:42Z
dc.date.available2022-07-06T15:56:42Z
dc.date.issued2021-07-15
dc.description(This article belongs to the Special Issue Compounds from Marine Sources as Hits and Leads for Pharmaceutical, Cosmeceutical and Industrial Applications)pt_PT
dc.description.abstractFucoxanthin (Fx) is a carotenoid derived from marine organisms that exhibits anticancer activities. However, its role as a potential drug adjuvant in breast cancer (BC) treatment is still poorly explored. Firstly, this study investigated the cytotoxic effects of Fx alone and combined with doxorubicin (Dox) and cisplatin (Cis) on a panel of 2D-cultured BC cell lines (MCF7, SKBR3 and MDA-MB-231) and one non-tumoral cell line (MCF12A). Fucoxanthin induced cytotoxicity against all the cell lines and potentiated Dox cytotoxic effects towards the SKBR3 and MDA-MB-231 cells. The combination triggering the highest cytotoxicity (Fx 10 µM + Dox 1 µM in MDA-MB-231) additionally showed significant induction of cell death and genotoxic effects, relative to control. In sequence, the same combination was tested on 3D cultures using a multi-endpoint approach involving bioactivity assays and microscopy techniques. Similar to 2D cultures, the combination of Fx and Dox showed higher cytotoxic effects on 3D cultures compared to the isolated compounds. Furthermore, this combination increased the number of apoptotic cells, decreased cell proliferation, and caused structural and ultrastructural damages on the 3D models. Overall, our findings suggest Fx has potential to become an adjuvant for Dox chemotherapy regimens in BC treatment.pt_PT
dc.description.sponsorshipThe Strategic Funding UIDB/04423/2020 and UIDP/04423/2020 partially supported this research, through national funds provided by FCT and ERDF to CIIMAR/CIMAR, in the framework of the program PT2020. The Doctoral Program in Biomedical Sciences, of the ICBAS-University of Porto, offered additional funds.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMolecules. 2021 Jul 15;26(14):4288. doi: 10.3390/molecules26144288.pt_PT
dc.identifier.doi10.3390/molecules26144288pt_PT
dc.identifier.issn1420-3049
dc.identifier.urihttp://hdl.handle.net/10400.18/8073
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationInterdisciplinary Centre of Marine and Environmental Research
dc.relationInterdisciplinary Centre of Marine and Environmental Research
dc.relation.publisherversionhttps://www.mdpi.com/1420-3049/26/14/4288pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCisplatinpt_PT
dc.subjectCombinatorial Therapypt_PT
dc.subjectDoxorubicinpt_PT
dc.subjectFucoxanthinpt_PT
dc.subjectSeaweed Compoundspt_PT
dc.subjectTriple-Negative Breast Cancerpt_PT
dc.titleFucoxanthin Holds Potential to Become a Drug Adjuvant in Breast Cancer Treatment: Evidence from 2D and 3D Cell Culturespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInterdisciplinary Centre of Marine and Environmental Research
oaire.awardTitleInterdisciplinary Centre of Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04423%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04423%2F2020/PT
oaire.citation.issue14pt_PT
oaire.citation.startPage4288pt_PT
oaire.citation.titleMoleculespt_PT
oaire.citation.volume26pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicatione47e16ab-4259-4ce8-af09-551f7f31443a
relation.isProjectOfPublicationc3064e67-096b-442c-b636-ae0e2da784a3
relation.isProjectOfPublication.latestForDiscoverye47e16ab-4259-4ce8-af09-551f7f31443a

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