Publication
Cell Model of Depression: Reduction of Cell Stress with Mirtazapine
| dc.contributor.author | Correia, Ana Salomé | |
| dc.contributor.author | Fraga, Sónia | |
| dc.contributor.author | Teixeira, João Paulo | |
| dc.contributor.author | Vale, Nuno | |
| dc.date.accessioned | 2022-11-03T12:39:55Z | |
| dc.date.available | 2022-11-03T12:39:55Z | |
| dc.date.issued | 2022-04-29 | |
| dc.description | This article belongs to the Special Issue Blood-Brain Barrier in CNS Injury and Repair 2022 | |
| dc.description.abstract | Depression is a very prevalent and complex disease. This condition is associated with a high rate of relapse, making its treatment a challenge. Thus, an intensive investigation of this disease and its treatment is necessary. In this work, through cell viability assays (MTT and neutral red assays) and alkaline comet assays, we aimed to test the induction of stress in human SH-SY5Y cells through the application of hydrocortisone and hydrogen peroxide and to test the reversal or attenuation of this stress through the application of mirtazapine to the cells. Our results demonstrated that hydrogen peroxide, and not hydrocortisone, can induce cellular stress, as evidenced by DNA damage and a global cellular viability reduction, which were alleviated by the antidepressant mirtazapine. The establishment of a cellular model of depression through stress induction is important to study new possibilities of treatment of this disease using cell cultures. | pt_PT |
| dc.description.sponsorship | This research was financed by FEDER—Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020—Operational Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, and by Portuguese funds through Fundação para a Ciência e a Tecnologia (FCT) in the framework of the project IF/00092/2014/CP1255/CT0004 and CHAIR in Onco-Innovation. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Int J Mol Sci . 2022 Apr 29;23(9):4942. doi: 10.3390/ijms23094942 | pt_PT |
| dc.identifier.doi | 10.3390/ijms23094942 | pt_PT |
| dc.identifier.issn | 1661-6596 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/8301 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | MDPI | pt_PT |
| dc.relation | New advance in biomedicine: peptide-mediated target drug delivery | |
| dc.relation.publisherversion | https://www.mdpi.com/1422-0067/23/9/4942 | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Depression | pt_PT |
| dc.subject | SH-SY5Y | pt_PT |
| dc.subject | Comet Assay | pt_PT |
| dc.subject | Mirtazapine | pt_PT |
| dc.subject | Hydrogen Peroxide | pt_PT |
| dc.subject | Stress | pt_PT |
| dc.subject | Tecnologias de Análise de DNA | pt_PT |
| dc.title | Cell Model of Depression: Reduction of Cell Stress with Mirtazapine | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | New advance in biomedicine: peptide-mediated target drug delivery | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00092%2F2014%2FCP1255%2FCT0004/PT | |
| oaire.citation.issue | 9 | pt_PT |
| oaire.citation.startPage | 4942 | pt_PT |
| oaire.citation.title | International Journal of Molecular Sciences | pt_PT |
| oaire.citation.volume | 23 | pt_PT |
| oaire.fundingStream | Investigador FCT | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.embargofct | Acesso de acordo com política editorial da revista. | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isProjectOfPublication | 6bbd9908-1ca6-47e7-9f84-3c2364f4c8fc | |
| relation.isProjectOfPublication.latestForDiscovery | 6bbd9908-1ca6-47e7-9f84-3c2364f4c8fc |