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Diversity of methicillin-resistant staphylococci among wild Lepus granatensis: first detection of mecA-MRSA in hares

2019-12-19Journal article Restricted access
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dc.contributor.authorSilva, Vanessa
dc.contributor.authorPereira, José Eduardo
dc.contributor.authorMaltez, Luís
dc.contributor.authorFerreira, Eugénia
dc.contributor.authorManageiro, Vera
dc.contributor.authorCaniça, Manuela
dc.contributor.authorCapelo, José L.
dc.contributor.authorIgrejas, Gilberto
dc.contributor.authorPoeta, Patrícia
dc.date.accessioned2020-04-22T15:30:55Z
dc.date.available2020-04-22T15:30:55Z
dc.date.issued2019-12-19
dc.description.abstractMRSA in humans, pets and livestock have been widely investigated, nevertheless, there is still little information of MRSA in wild animals. Therefore, this study aimed to investigate the occurrence and antimicrobial resistance profiles of methicillin-resistant staphylococci (MRS) in wild Iberian hares and to characterize their genetic lineages. Samples from 83 wild hares (Lepus granatensis) were collected during the hunting season. Isolation of MRS was accomplish using Oxacillin Resistant Screening Agar medium with 2 mg/L of oxacillin. The susceptibility of the isolates was tested by the Kirby-Bauer disc diffusion method. The presence of resistance and virulence genes was studied by PCR. S. aureus strains were further characterized by multilocus sequence typing, agr, spa and SCCmec typing. From the 83 samples, 12 (14.45%) coagulase-negative staphylococci and 3 (3.6%) MRSA strains were isolated. Nine coagulase-negative isolates showed resistance to at least one antibiotic. One MRSA isolate showed a multidrug-resistant profile with resistances to ß-lactams, aminoglycosides, macrolides and lincosamides. All MRSA strains were ascribed to ST2855, t1190 and SCCmec type III. The frequency of MRSA strains in wild hares was low, nevertheless, the presence of MRSA in game animals is considered a public health problem and may represent a route of transmission between animals and humans.pt_PT
dc.description.sponsorshipThis work was funded by the R&D Project CAREBIO2 - Comparative assessment of antimicrobial resistance in environmental biofilms through proteomics - towards innovative theranostic biomarkers, with reference NORTE-01-0145-FEDER-030101 and PTDC/SAU-INF/30101/2017, financed by the European Regional Development Fund (ERDF) through the Northern Regional Operational Program (NORTE 2020) and the Foundation for Science and Technology (FCT). This work was supported by the Associate Laboratory for Green Chemistry-LAQV which is financed by national funds from FCT/MCTES (UID/QUI/50006/2019). Vanessa Silva is supported by national funds through FCT/MCTES and by the European Social Fund through POCH/FSE under the PhD grant SFRH/BD/137947/2018.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFEMS Microbiol Ecol . 2020 Feb;96(1):fiz204. Epub: 2019 Dec 19. doi: 10.1093/femsec/fiz204pt_PT
dc.identifier.doi10.1093/femsec/fiz204pt_PT
dc.identifier.issn0168-6496
dc.identifier.urihttp://hdl.handle.net/10400.18/6483
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherOxford University Press
dc.relation.publisherversionhttps://academic.oup.com/femsec/article-abstract/96/1/fiz204/5681390?redirectedFrom=fulltextpt_PT
dc.subjectMRSApt_PT
dc.subjectmecApt_PT
dc.subjectIberian Harept_PT
dc.subjectWildlifept_PT
dc.subjectMethicillin-resistant Staphylococcipt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.titleDiversity of methicillin-resistant staphylococci among wild Lepus granatensis: first detection of mecA-MRSA in harespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.citation.issue1pt_PT
oaire.citation.startPagefiz204pt_PT
oaire.citation.titleFEMS Microbiology Ecologypt_PT
oaire.citation.volume96pt_PT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctDe acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationd7883dc7-6335-4f26-8bd0-e2ea4bb07c07
relation.isProjectOfPublication.latestForDiscoveryd7883dc7-6335-4f26-8bd0-e2ea4bb07c07

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