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Magnetite nanoparticles functionalized propolis extract as nano-antibiotics against methicillin resistant strains of Staphylococcus aureus

2018-02Conference object Restricted access
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dc.contributor.authorEl-Guendouz, Soukaina
dc.contributor.authorLyoussi, Badiaa
dc.contributor.authorLourenço, João P.
dc.contributor.authorRosa Costa, Ana M.
dc.contributor.authorMiguel, Maria G.
dc.contributor.authorDias, Cristina M.B.
dc.contributor.authorManhita, Ana C.
dc.contributor.authorLuísa, Jordão
dc.contributor.authorNogueira, Isabel
dc.contributor.authorFaleiro, Maria Leonor
dc.date.accessioned2019-02-06T12:08:22Z
dc.date.available2022-01-01T01:30:10Z
dc.date.issued2018-02
dc.description.abstractDue to their ability to penetrate planktonic and biofilm organized bacteria , nano-antimicrobial agents have been perceived, in recent years, in almost every domain of life especially in health care for the control of infectious diseases. Magnetite nanoparticles (MNPs) have been evaluated for inhibiting microbial growth and biofilm formation. The aim of this study was to evaluate the effect of Moroccan propolis extract functionalized magnetite nanoparticles as nanoantibiotics against methicillin resistant Staphylococcus aureus (MRSA) growth. Chemical composition of propolis was established by pyrolysis GC-MS method. The fabricated nanostructure was characterized by X-ray Diffraction, Transmission Electron Microscopy, and Fourier Transform-Infrared Spectroscopy. FTIR spectroscopy data allowed to confirm functionalization of the MNPs upon contact with oleic acid (OA). Further functionalization with either propolis extract or chloramphenicol was also confirmed by observation of the corresponding FTIR spectra. When both components were added together to OA-functionalized MNPs, the presence of the former is clear whereas that of the latter is less evident, although possible. The use of MNPs functionalized with propolis and chloramphenicol (positive control) at MIC value against methicillin susceptible S. aureus (MSSA) and MRSA strains inhibited the bacterial growth. After the treatment with functionalized MNPs the cells showed disruption of the cell wall as assessed by transmission electron microscopy (TEM). The use of the combination of propolis and the chloramphenicol antibiotic in free form at minimum inhibitory concentration largely impaired both MSSA and MRSA strains, after 2 h of treatment no viable cells of MRSA 2 and MRSA 16 were recovered.pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/5708
dc.languagepor
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectMagnetite Nanoparticulespt_PT
dc.subjectS. aureuspt_PT
dc.subjectStaphylococcus aureuspt_PT
dc.subjectElectron Microscopypt_PT
dc.subjectPropolispt_PT
dc.subjectNanopartículas de Magnetitept_PT
dc.subjectMNPspt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleMagnetite nanoparticles functionalized propolis extract as nano-antibiotics against methicillin resistant strains of Staphylococcus aureuspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceCoimbra, Portugalpt_PT
oaire.citation.titleV Congresso Iberico de Apicultura, 1-3 fevereiro 2018pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT

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