Repository logo
 
Publication

Genotoxic effects of occupational exposure to lead and influence of polymorphisms in genes involved in lead toxicokinetics and in DNA repair

2012-08Journal article Restricted access
Simple item page
dc.contributor.authorGarcía-Lestón, Julia
dc.contributor.authorRoma-Torres, Joana
dc.contributor.authorVilares, Maria
dc.contributor.authorPinto, Rui
dc.contributor.authorPrista, João
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorMayan, Olga
dc.contributor.authorConde, Joao
dc.contributor.authorPingarilho, Marte
dc.contributor.authorGaspar, Jorge Francisco
dc.contributor.authorPásaro, Eduardo
dc.contributor.authorMéndez, Josefina
dc.contributor.authorLaffon, Blanca
dc.date.accessioned2013-02-11T11:44:27Z
dc.date.available2013-02-11T11:44:27Z
dc.date.issued2012-08
dc.description.abstractLead is still widely used in many industrial processes and is very persistent in the environment. Although toxic effects caused by occupational exposure to lead have been extensively studied, there are still conflicting results regarding its genotoxicity. In a previous pilot study we observed some genotoxic effects in a population of lead exposed workers. Thus, we extended our study analysing a larger population, increasing the number of genotoxicity endpoints, and including a set of 20 genetic polymorphisms related to lead toxicokinetics and DNA repair as susceptibility biomarkers. Our population comprised 148 workers from two Portuguese factories and 107 controls. The parameters analysed were: blood lead levels (BLL) and δ-aminolevulinic acid dehydratase (ALAD) activity as exposure biomarkers, and T-cell receptor (TCR) mutation assay, micronucleus (MN) test, comet assay and OGG1-modified comet assay as genotoxicity biomarkers. Lead exposed workers showed markedly higher BLL and lower ALAD activity than the controls, and significant increases of TCR mutation frequency (TCR-Mf), MN rate and DNA damage. Oxidative damage did not experience any significant alteration in the exposed population. Besides, significant influence was observed for VDR rs1544410 polymorphism on BLL; APE1 rs1130409 and LIG4 rs1805388 polymorphisms on TCR-Mf; MUTYH rs3219489, XRCC4 rs28360135 and LIG4 rs1805388 polymorphisms on comet assay parameter; and OGG1 rs1052133 and XRCC4 rs28360135 polymorphisms on oxidative damage. Our results showed genotoxic effects related to occupational lead exposure to levels under the Portuguese regulation limit of 70 μg/dl. Moreover, a significant influence of polymorphisms in genes involved in DNA repair on genotoxicity biomarkers was observed.por
dc.identifier.citationEnviron Int. 2012 Aug;43:29-36. Epub 2012 Mar 30por
dc.identifier.issn0160-4120
dc.identifier.otherdoi: 10.1016/j.envint.2012.03.001
dc.identifier.urihttp://hdl.handle.net/10400.18/1217
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevier (Pergamon)por
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0160412012000505por
dc.subjectLeadpor
dc.subjectGenotoxicitypor
dc.subjectT-cell Receptor Mutation Assaypor
dc.subjectMicronucleus Testpor
dc.subjectComet Assaypor
dc.subjectGenetic Polymorphismspor
dc.subjectAr e Saúde Ocupacionalpor
dc.subjectGenotoxicidade Ambientalpor
dc.titleGenotoxic effects of occupational exposure to lead and influence of polymorphisms in genes involved in lead toxicokinetics and in DNA repairpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage36por
oaire.citation.startPage29por
oaire.citation.titleEnvironment Internationalpor
oaire.citation.volume43por
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
2012 Environment International JULIA.pdf
Size:
388.54 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

DSA - Artigos em revistas internacionais