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Combined detection of molecular and serological signatures of viral infections: The dual assay concept

2022-08-15Journal article Restricted access
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dc.contributor.authorAlbuquerque, D. C.
dc.contributor.authorMartins, V. C.
dc.contributor.authorFernandes, E.
dc.contributor.authorZé-Zé, L.
dc.contributor.authorAlves, M.J.
dc.contributor.authorCardoso, S.
dc.date.accessioned2023-01-10T15:16:30Z
dc.date.available2023-01-10T15:16:30Z
dc.date.issued2022-08-15
dc.description.abstractThe recent worldwide spread of viral infections has highlighted the need for accurate, fast, and inexpensive disease diagnosis and monitorization methods. Current diagnostics tend to focus either on molecular or serological testing. In this work we propose a dual detection assay approach for viral diseases, where both serological and molecular assays are combined in a single analysis performed on a magnetoresistive system. This type of assay guarantees an accurate assessment of the infection phase, saving time and costs associated with multiple independent tests. Zika and dengue viruses were used as model diseases for the validation of the system. Human IgG anti-zika and anti-dengue antibodies were successfully detected in infected patients' serum, using a novel approach combining competitive and sandwich strategies in a magnetoresistive portable platform. Specificity and sensitivity values of 100% were obtained. Calibration curves with dynamic ranges between 10 ng/mL and 1 μg/mL were established achieving LODs of 1.26 and 1.38 nM for IgG anti-ZIKV and anti-DENV antibodies, respectively. Viral RNA detection down to a few hundreds of pM was also successfully carried out after the design of specific oligo probes and primers for RT-PCR amplification. Dual assays were performed for both viruses, where viral RNA and anti-virus antibodies in serum samples were simultaneously detected. The results obtained for the detection of the molecular and serological targets in the dual assay format show no significant difference between the ones obtained individually, proving the feasibility and accuracy of the dual detection assay. This assay format represents a new paradigm in viral infections diagnostics.pt_PT
dc.description.abstractHighlights: Dual assay for combined detection of RNA and antibodies in viral infections; Zika and dengue viral RNA detection down to a few pM; Successful detection of IgG anti-Zika and anti-dengue antibodies in serum samples, with an LOD of 1.26 and 1.38 nM, respectively; Dual assay is comparable to individual assays, proving its feasibility in clinical setting.pt_PT
dc.description.sponsorshipThe authors wish to acknowledge the Fundação para a Ciência e a Tecnologia for funding of the Research Unit INESC MN (UID/05367/2020) through pluriannual BASE and PROGRAMATICO financing. The work was supported by Phage4BacID project reference LISBOA-01-247-FEDER-33603 funded by P2020, Fim4Stroke project reference PTDC-MEC-URG-29561-2017 funded by FCT and Moore4Medical project funded by the ECSEL Joint Undertaking under grant number H2020-ECSEL-2019-IA-876190. Acknowledgment is also due to FCT grant with ref PD/BD/143024/2018 for Doctoral Program AIM-Advanced Integrated Microsystems.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBiosens Bioelectron. 2022 Aug 15;210:114302. doi: 10.1016/j.bios.2022.114302. Epub 2022 Apr 25.pt_PT
dc.identifier.doi10.1016/j.bios.2022.114302pt_PT
dc.identifier.issn0956-5663
dc.identifier.urihttp://hdl.handle.net/10400.18/8417
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationINESC Microsystems and Nanotechnologies
dc.relationINESC Microsystems and Nanotechnologies
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0956566322003426?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectMagnetoresistive Biosensorpt_PT
dc.subjectDual Assaypt_PT
dc.subjectMolecular Testingpt_PT
dc.subjectImmunoassayspt_PT
dc.subjectViral Infectionspt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleCombined detection of molecular and serological signatures of viral infections: The dual assay conceptpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleINESC Microsystems and Nanotechnologies
oaire.awardTitleINESC Microsystems and Nanotechnologies
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F05367%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F05367%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/PD%2FBD%2F143024%2F2018/PT
oaire.citation.startPage114302pt_PT
oaire.citation.titleBiosensors and Bioelectronicspt_PT
oaire.citation.volume210pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationbaf645dc-796c-498d-b8c9-06c872481314
relation.isProjectOfPublicatione5b48d7a-19c4-4bdd-a9c6-9475f06b5c52
relation.isProjectOfPublicationd90077a3-a3ad-43f7-adb1-245b5e56e1d6
relation.isProjectOfPublication.latestForDiscoverybaf645dc-796c-498d-b8c9-06c872481314

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