Repository logo
 
Publication

Applicability of In Vitro Methods to Study Patulin Bioaccessibility and Its Effects on Intestinal Membrane Integrity

2014-07-29Journal article Restricted access
Simple item page
dc.contributor.authorAssunção, Ricardo
dc.contributor.authorFerreira, Mariana
dc.contributor.authorMartins, Carla
dc.contributor.authorDiaz, Irene
dc.contributor.authorPadilla, Beatriz
dc.contributor.authorDupond, Didier
dc.contributor.authorBragança, Mauro
dc.contributor.authorAlvito, Paula
dc.date.accessioned2015-01-27T15:55:26Z
dc.date.available2015-01-27T15:55:26Z
dc.date.issued2014-07-29
dc.description.abstractIn human health risk assessment, ingestion of food is considered a major route of exposure to many contaminants, although the total amount of an ingested contaminant (external dose) does not always reflect the quantity available for the body (internal dose). In this study, two in vitro methods were applied to study bioaccessibility and intestinal membrane integrity of cells exposed to patulin, a mycotoxin with significant public health risk. Seven artificially contaminated fruit juices were assayed in the presence or absence of a standard meal, showing a significant difference for bioaccessibility values between contaminated samples alone (mean 27.65 ± 13.50%) and combinations with a standard meal (mean 7.89 ± 4.03%). Different concentrations of patulin (PAT) and cysteine (CYS) (protector agent) were assayed in Caco-2 cells monolayers. At 95 μM, PAT produced a marked decrease in transepithelial electrical resistance (TEER). This effect was significantly reduced when 400 μM and 4000 μM CYS was added to the cells. Combined use of in vitro digestion models with other techniques using intestinal cell lines, such as in vitro intestinal absorption models that use Caco-2 cells, may offer a more comprehensive model of what is occurring during digestion and absorption processes. The study of beneficial effects of protective agents would also be enhanced.por
dc.description.sponsorshipFCTpor
dc.identifier.citationJ Toxicol Environ Health A. 2014;77(14-16):983-92. doi: 10.1080/15287394.2014.911138por
dc.identifier.doi10.1080/15287394.2014.911138
dc.identifier.issn1528-7394
dc.identifier.urihttp://hdl.handle.net/10400.18/2697
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherTaylor & Francis: STM, Behavioural Science and Public Health Titlespor
dc.relationMycomixpor
dc.relation.publisherversionhttp://www.tandfonline.com/doi/full/10.1080/15287394.2014.911138#tabModulepor
dc.subjectSegurança Alimentarpor
dc.subjectToxicologiapor
dc.subjectModelos de Digestão in vitropor
dc.titleApplicability of In Vitro Methods to Study Patulin Bioaccessibility and Its Effects on Intestinal Membrane Integritypor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage992por
oaire.citation.startPage983por
oaire.citation.titleJournal of Toxicology and Environmental Health, Part A: Current Issuespor
oaire.citation.volume77(14-16)por
rcaap.rightsembargoedAccesspor
rcaap.typearticlepor

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
2014 Applicability of In Vitro Methods to Study Patulin.pdf
Size:
213.74 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

DAN - Artigos em revistas internacionais