Publication
Multidrug-Resistant Salmonella enterica Serovar Rissen Clusters Detected in Azores Archipelago, Portugal
| dc.contributor.author | Silveira, Leonor | |
| dc.contributor.author | Pinto, Miguel | |
| dc.contributor.author | Isidro, Joana | |
| dc.contributor.author | Pista, Ângela | |
| dc.contributor.author | Themudo, Patrícia | |
| dc.contributor.author | Vieira, Luís | |
| dc.contributor.author | Machado, Jorge | |
| dc.contributor.author | Gomes, João Paulo | |
| dc.date.accessioned | 2020-04-28T14:18:57Z | |
| dc.date.available | 2020-04-28T14:18:57Z | |
| dc.date.issued | 2019-12-14 | |
| dc.description | Free PMC article: https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/31950026/ | pt_PT |
| dc.description.abstract | Gastrointestinal infections caused by nontyphoidal Salmonella (NTS) remain one of the main causes of foodborne illness worldwide. Within the multiple existing Salmonella enterica serovars, the serovar Rissen is rarely reported, particularly as a cause of human salmonellosis. Between 2015 and 2017, the Portuguese National Reference Laboratory of Gastrointestinal Infections observed an increase in the number of clinical cases caused by multidrug-resistant (MDR) S. enterica serovar Rissen, particularly from the Azores archipelago. In the present study, we analyzed by whole genome sequencing (WGS) all clinical, animal, food, and environmental isolates received up to 2017 in the Portuguese Reference Laboratories. As such, through a wgMLST-based gene-by-gene analysis, we aimed to identify potential epidemiological clusters linking clinical and samples from multiple sources, while gaining insight into the genetic diversity of S. enterica serovar Rissen. We also investigated the genetic basis driving the observed multidrug resistance. By integrating 60 novel genomes with all publicly available serovar Rissen genomes, we observed a low degree of genetic diversity within this serovar. Nevertheless, the majority of Portuguese isolates showed high degree of genetic relatedness and a potential link to pork production. An in-depth analysis of these isolates revealed the existence of two major clusters from the Azores archipelago composed of MDR isolates, most of which were resistant to at least five antimicrobials. Considering the well-known spread of MDR between gastrointestinal bacteria, the identification of MDR circulating clones should constitute an alert to public health authorities. Finally, this study constitutes the starting point for the implementation of the "One Health" approach for Salmonella surveillance in Portugal. | pt_PT |
| dc.description.sponsorship | This work was partially funded by the GenomePT project (POCI-01-0145- FEDER-022184), supported by COMPETE 2020–Operational Programme for Competitiveness and Internationalisation (POCI), Lisboa Portugal Regional Operational Programme (Lisboa2020), and Algarve Portugal Regional Operational Programme (CRESC Algarve2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) and by the Fundação para a Ciência e a Tecnologia (FCT) | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Int J Genomics. 2019 Dec 14;2019:1860275. doi: 10.1155/2019/1860275. eCollection 2019. [Epub ahead of print] | pt_PT |
| dc.identifier.doi | 10.1155/2019/1860275 | pt_PT |
| dc.identifier.issn | 2314-436X | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/6538 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Hindawi | pt_PT |
| dc.relation.publisherversion | https://www.hindawi.com/journals/ijg/2019/1860275/ | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Salmonella | pt_PT |
| dc.subject | Nontyphoidal Salmonella | pt_PT |
| dc.subject | Salmonella enterica | pt_PT |
| dc.subject | Gastrointestinal Infections | pt_PT |
| dc.subject | Azores Archipelago | pt_PT |
| dc.subject | Portugal | pt_PT |
| dc.subject | Infecções Gastrointestinais | pt_PT |
| dc.title | Multidrug-Resistant Salmonella enterica Serovar Rissen Clusters Detected in Azores Archipelago, Portugal | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.startPage | 1860275 | pt_PT |
| oaire.citation.title | International Journal of Genomics | pt_PT |
| oaire.citation.volume | 2019 | pt_PT |
| rcaap.embargofct | De acordo com política editorial da revista. | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |