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Bioguided Identification of Active Antimicrobial Compounds from Asphodelus bento-rainhae and Asphodelus macrocarpus Root Tubers

2023-06-01Journal article Open access
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dc.contributor.authorMalmir, Maryam
dc.contributor.authorLima, Katelene
dc.contributor.authorCamões, Sérgio Póvoas
dc.contributor.authorManageiro, Vera
dc.contributor.authorDuarte, Maria Paula
dc.contributor.authorMiranda, Joana Paiva
dc.contributor.authorSerrano, Rita
dc.contributor.authorda Silva, Isabel Moreira
dc.contributor.authorLima, Beatriz Silva
dc.contributor.authorCaniça, Manuela
dc.contributor.authorSilva, Olga
dc.date.accessioned2024-01-25T11:30:44Z
dc.date.available2024-01-25T11:30:44Z
dc.date.issued2023-06-01
dc.description.abstractRoot tubers of Asphodelus bento-rainhae subsp. bento-rainhae (AbR), a vulnerable endemic species, and Asphodelus macrocarpus subsp. macrocarpus (AmR) have traditionally been used in Portugal to treat inflammatory and infectious skin disorders. The present study aims to evaluate the in vitro antimicrobial activity of crude 70% and 96% hydroethanolic extracts of both medicinal plants, specifically against multidrug-resistant skin-related pathogens, to identify the involved marker secondary metabolites and also to assess the pre-clinical toxicity of these medicinal plant extracts. Bioguided fractionation of the 70% hydroethanolic extracts of both species using solvents of increasing polarity, namely diethyl ether (DEE: AbR-1, AmR-1), ethyl acetate (AbR-2, AmR-2) and aqueous (AbR-3, AmR-3) fractions, enabled the identification of the DEE fractions as the most active against all the tested Gram-positive microorganisms (MIC: 16 to 1000 µg/mL). Furthermore, phytochemical analyses using TLC and LC-UV/DAD-ESI/MS techniques revealed the presence of anthracene derivatives as the main constituents of DEE fractions, and five known compounds, namely 7'-(chrysophanol-4-yl)-chrysophanol-10'-C-beta-D-xylopyranosyl-anthrone (p), 10,7'-bichrysophanol (q), chrysophanol (r), 10-(chrysophanol-7'-yl)-10-hydroxychrysophanol-9-anthrone (s) and asphodelin (t), were identified as the main marker compounds. All these compounds showed high antimicrobial activity, particularly against Staphylococcus epidermidis (MIC: 3.2 to 100 µg/mL). Importantly, no cytotoxicity against HepG2 and HaCaT cells (up to 125 µg/mL) for crude extracts of both species and genotoxicity (up to 5000 µg/mL, with and without metabolic activation) for AbR 96% hydroethanolic extract was detected using the MTT and Ames tests, respectively. Overall, the obtained results contribute to the concrete validation of the use of these medicinal plants as potential sources of antimicrobial agents in the treatment of skin diseases.pt_PT
dc.description.sponsorshipThis research was funded by the Foundation for Science and Technology/MCTES (FCT, Portugal) through national funds to iMed.ULisboa (UIDP/04138/2020, UIDB/04138/2020), to CECA (UIDB/00211/2020) and to MEtRICs (UIDP/04077/2020, UIDB/04077/2020) research projects, as well as a doctoral scholarship (SFRH/BD/125310/2016) granted to the first authorpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPharmaceuticals (Basel). 2023 Jun 1;16(6):830. doi: 10.3390/ph16060830pt_PT
dc.identifier.doi10.3390/ph16060830pt_PT
dc.identifier.issn1424-8247
dc.identifier.urihttp://hdl.handle.net/10400.18/8979
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationResearch Institute for Medicines
dc.relationCenter for the Study of Animal Science
dc.relationMechanical Engineering and Resource Sustainability Center
dc.relationMechanical Engineering and Resource Sustainability Center
dc.relationDiscovery of new antimicrobial drugs active against resistant pathogen agents: The case study of Portuguese Asphodelus species
dc.relation.publisherversionhttps://www.mdpi.com/1424-8247/16/6/830pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAsphodelus bento-rainhaept_PT
dc.subjectAsphodelus macrocarpuspt_PT
dc.subjectAnthracene Derivativespt_PT
dc.subjectAntimicrobial Activitypt_PT
dc.subjectRoot Tuberspt_PT
dc.subjectToxicitypt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.titleBioguided Identification of Active Antimicrobial Compounds from Asphodelus bento-rainhae and Asphodelus macrocarpus Root Tuberspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleCenter for the Study of Animal Science
oaire.awardTitleMechanical Engineering and Resource Sustainability Center
oaire.awardTitleMechanical Engineering and Resource Sustainability Center
oaire.awardTitleDiscovery of new antimicrobial drugs active against resistant pathogen agents: The case study of Portuguese Asphodelus species
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04138%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00211%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04077%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04077%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F125310%2F2016/PT
oaire.citation.issue6pt_PT
oaire.citation.startPage830pt_PT
oaire.citation.titlePharmaceuticalspt_PT
oaire.citation.volume16pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com a política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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