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Ceruloplasmin expression by human peripheral blood lymphocytes: a new link between immunity and iron metabolism

2008-02Journal article Open access
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dc.contributor.authorBanha, J.
dc.contributor.authorMarques, L.
dc.contributor.authorOliveira, R.
dc.contributor.authorMartins, M. de F.
dc.contributor.authorPaixão, E.
dc.contributor.authorPereira, D.
dc.contributor.authorMalhó, R.
dc.contributor.authorPenque, D.
dc.contributor.authorCosta, L.
dc.date.accessioned2011-11-09T15:46:43Z
dc.date.available2011-11-09T15:46:43Z
dc.date.issued2008-02
dc.description.abstractCeruloplasmin (CP) is a multicopper oxidase involved in the acute phase reaction to stress. Although the physiological role of CP is uncertain, its role in iron (Fe) homeostasis and protection against free radical-initiated cell injury has been widely documented. Previous studies showed the existence of two molecular isoforms of CP: secreted CP (sCP) and a membrane glycosylphosphatidylinositol (GPI)-anchored form of CP (GPI-CP). sCP is produced mainly by the liver and is abundant in human serum whereas GPI-CP is expressed in mammalian astrocytes, rat leptomeningeal cells, and Sertolli cells. Herein, we show using RT-PCR that human peripheral blood lymphocytes (huPBL) constitutively express the transcripts for both CP molecular isoforms previously reported. Also, expression of CP in huPBL is demonstrated by immunofluorescence with confocal microscopy and flow cytometry analysis using cells isolated from healthy blood donors with normal Fe status. Importantly, the results obtained show that natural killer cells have a significantly higher CP expression compared to all other major lymphocyte subsets. In this context, the involvement of lymphocyte-derived CP on host defense processes via its anti/prooxidant properties is proposed, giving further support for a close functional interaction between the immune system and the Fe metabolism.por
dc.identifier.citationFree Radic Biol Med. 2008 Feb 1;44(3):483-92. Epub 2007 Oct 22.por
dc.identifier.issn0891-5849
dc.identifier.otherdoi:10.1016/j.freeradbiomed.2007.10.032
dc.identifier.urihttp://hdl.handle.net/10400.18/315
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S089158490700682Xpor
dc.subjectCeruloplasminpor
dc.subjectPeripheral blood lymphocytespor
dc.subjectIronpor
dc.subjectCopperpor
dc.subjectNK cellspor
dc.subjectDeterminantes Imunológicos em Doenças Crónicaspor
dc.titleCeruloplasmin expression by human peripheral blood lymphocytes: a new link between immunity and iron metabolismpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage492por
oaire.citation.startPage483por
oaire.citation.titleFree Radical Biology and Medicinepor
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

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