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Nano-bio interactions of titanium dioxide nanomaterials in the intestinal moiety after simulated digestion in vitro

2020-01-20Conference object Restricted access
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dc.contributor.authorLouro, Henriqueta
dc.contributor.authorRolo, Dora
dc.contributor.authorGramacho, Ana C.
dc.contributor.authorPereira, Joana
dc.contributor.authorMatos, Paulo
dc.contributor.authorJordan, Peter
dc.contributor.authorMartins, Carla
dc.contributor.authorAssunção, Ricardo
dc.contributor.authorAlvito, Paula
dc.contributor.authorGonçalves, Lídia
dc.contributor.authorBettencourt, Ana F.
dc.contributor.authorSilva, Maria João
dc.date.accessioned2021-03-13T09:37:05Z
dc.date.available2021-03-13T09:37:05Z
dc.date.issued2020-01-20
dc.description.abstractThe exponential development of nanomaterials (NMs) contrasts with insufficient risk assessment for human health and the environment, leading to concerns for public health. Their potential to improve many products and processes, namely in agriculture, food and feed industry, has led to increased use of NMs, such as titanium dioxide nanomaterials (TiO2). In fact, oral exposure to TiO2 may occur either directly, through the consumption of products/pharmaceuticals containing NMs, or indirectly, through the ingestion of food/water contaminated due to environmental accumulation. Therefore, the gastrointestinal tract (GIT) appears to be a probable route of exposure to NMs and may lead to systemic exposure if the body barriers are surpassed. Since NMs primary physicochemical properties may define nano-bio interactions1, NMs with the same chemistry but with different shape, diameter, length, surface charge or functionalization may lead to different toxicities. Conversely, since the GIT is chemically and physically complex, ingested NMs will pass through different environments prior to their intestinal uptake, affecting their physicochemical properties, so that these secondary physicochemical properties should be considered while evaluating their safety in the food chain. The aim of this work was to investigate the nano-bio interactions of three TiO2 (NM-102, NM-103, NM-105, from Joint Research Centre, Ispra) in the context of intestinal tract and digestion processes. As alternative to animal testing, an in vitro harmonized digestion method2 was used for simulating the human digestion of NMs and their characteristics were studied in acellular and intestinal (Caco-2) cells context. The NMs were characterized using dynamic light scattering (DLS, for size distribution), electrophoretic light scattering (zeta potential), transmission electron microscopy (TEM) for morphological characterization and X-ray energy dispersive spectrometry (EDS) for elemental chemical analysis. TiO2 samples were labelled directly with a fluorescent probe and used for uptake studies with confocal microscopy (CM). In parallel, a co-culture model of Caco-2 with mucous-secreting HT29-MTX was initiated and exposed to TiO2 to ascertain epithelial barrier translocation. The results did not show major differences in the NMs’ pH, osmolality, charge signal or mean size, except for NM-103. TEM and TEM –EDS analysis showed that it is possible to identify primary particles after digestion process and preliminary CM results suggest that some of the tested TiO2 nanomaterials can be uptake by the cells, suggestive of potential subcellular effects that warrant further investigation. References [1] H. Louro, A. Saruga, J. Santos, M. Pinhão, M.J. Silva, Toxicol. In Vitro. 56 (2019)172–183. [2] A. Brodkorb, L.Egger, M. Alminger, P. Alvito et al. Nat Protoc. 2019 Apr;14(4):991-1014.pt_PT
dc.description.sponsorshipFunded by FCT/MCTES through national funds (PIDDAC), PTDC/SAU-PUB/29481/2017 and co-funded by UID/BIM/00009/2013 (Centre for Toxicogenomics and Human Health – ToxOmics, FCT), iMed.ULisboa (Pest-UID/DTP/04138/2018) and CESAM (UID/ AMB/50017/2019).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationHenriqueta Louro, Dora Rolo, Ana C. Gramacho, Joana Pereira, Paulo Matos, Peter Jordan, Carla Martins, Ricardo Assunção, Paula Alvito, Lídia Gonçalves, Ana F. Bettencourt, Maria João Silva.2020. Nano-bio interactions of titanium dioxide nanomaterials in the intestinal moiety after simulated digestion in vitro. IVth International Caparica Symposium on Nanoparticles, Nanomaterials and Applications 2020 (ISN2A), 20 January 2020, Caparica, Portugal. Oral communication.pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/7434
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationPest-UID/DTP/04138/2018pt_PT
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectIngested Nanomaterialspt_PT
dc.subjectNanotoxicologypt_PT
dc.subjectFood Safetypt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.subjectSegurança Alimentarpt_PT
dc.titleNano-bio interactions of titanium dioxide nanomaterials in the intestinal moiety after simulated digestion in vitropt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-PUB%2F29481%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIM%2F00009%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAMB%2F50017%2F2013/PT (2019)
oaire.citation.conferencePlaceCaparica, Portugalpt_PT
oaire.citation.title4th International Caparica Symposium on Nanoparticles, Nanomaterials and Applications (ISN2A), 20–23 January 2020pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
oaire.fundingStream5876
person.familyNameLouro
person.familyNameSilva
person.givenNameHenriqueta
person.givenNameMaria Joao
person.identifier157627
person.identifier.ciencia-id721D-2BB1-7DB1
person.identifier.ciencia-id7710-643D-97A3
person.identifier.orcid0000-0001-9744-7332
person.identifier.orcid0000-0002-6060-0716
person.identifier.scopus-author-id6507971479
person.identifier.scopus-author-id55944437600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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relation.isAuthorOfPublication.latestForDiscovery2361a951-8b9a-4b90-92d6-f6384003a242
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