Publication
Resistance of mRNAs with AUG-proximal nonsense mutations to nonsense-mediated decay reflects variables of mRNA structure and translational activity.
| dc.contributor.author | Pereira, Francisco | |
| dc.contributor.author | Kong, Jian | |
| dc.contributor.author | Siva, Ana Luísa | |
| dc.contributor.author | Liebhaber, Stephen A | |
| dc.contributor.author | Romão, Luísa | |
| dc.date.accessioned | 2016-02-16T17:10:07Z | |
| dc.date.available | 2016-02-16T17:10:07Z | |
| dc.date.issued | 2015-07-27 | |
| dc.description | Acessível em: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4513866/ | pt_PT |
| dc.description.abstract | Nonsense-mediated mRNA decay (NMD) is a surveillance pathway that recognizes and selectively degrades mRNAs carrying premature termination codons (PTCs). The level of sensitivity of a PTC-containing mRNA to NMD is multifactorial. We have previously shown that human β-globin mRNAs carrying PTCs in close proximity to the translation initiation AUG codon escape NMD. This was called the 'AUG-proximity effect'. The present analysis of nonsense codons in the human α-globin mRNA illustrates that the determinants of the AUG-proximity effect are in fact quite complex, reflecting the ability of the ribosome to re-initiate translation 3' to the PTC and the specific sequence and secondary structure of the translated ORF. These data support a model in which the time taken to translate the short ORF, impacted by distance, sequence, and structure, not only modulates translation re-initiation, but also impacts on the exact boundary of AUG-proximity protection from NMD. | pt_PT |
| dc.identifier.citation | Nucleic Acids Res. 2015 Jul 27;43(13):6528-44. doi: 10.1093/nar/gkv588. Epub 2015 Jun 11. | pt_PT |
| dc.identifier.doi | 10.1093/nar/gkv588 | pt_PT |
| dc.identifier.issn | 0305-1048 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/3346 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Oxford University Press | pt_PT |
| dc.relation.publisherversion | http://nar.oxfordjournals.org/cgi/pmidlookup?view=long&pmid=26068473 | pt_PT |
| dc.subject | Expressão Génica | pt_PT |
| dc.subject | Genómica Funcional e Estrutural | pt_PT |
| dc.title | Resistance of mRNAs with AUG-proximal nonsense mutations to nonsense-mediated decay reflects variables of mRNA structure and translational activity. | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 6544 | pt_PT |
| oaire.citation.startPage | 6528 | pt_PT |
| oaire.citation.title | Nucleic Acids Research | pt_PT |
| oaire.citation.volume | 43(13) | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |