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Cytotoxicity Assessment of Novel Cellulose Nanomaterials on Intestinal Cells

2021-09-27Conference object Restricted access
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dc.contributor.authorVital, Nádia
dc.contributor.authorPinto, Fátima
dc.contributor.authorRolo, Dora
dc.contributor.authorPedrosa, Jorge
dc.contributor.authorFerreira, Paulo J.T.
dc.contributor.authorKranendonk, Michel
dc.contributor.authorSilva, Maria João
dc.contributor.authorLouro, Henriqueta
dc.date.accessioned2022-01-13T17:39:06Z
dc.date.available2022-01-13T17:39:06Z
dc.date.issued2021-09-27
dc.description.abstractCellulose nanomaterials (CNMs) emerged as an important group of nanomaterials with potential applications in multiple food-related products, as zero-calorie filler/thickener/stabilizer, or as a substitute for petroleum-based food packaging materials. Human oral exposure to CNMs is increasing, but little is known about the potential adverse biological impact of CNMs on human gastrointestinal tract. To contribute to the development of innovative CNMs for the food sector and to their early-stage safety assessment, in this work new CNMs were prepared, and their cytotoxic effects were investigated in human intestinal cell lines. Two cellulose micro/nanofibrillated (CNFs), were synthetized from industrial bleached Eucalyptus globulus kraft pulp using different pretreatments (enzymatic and TEMPO-mediated oxidation) followed by a high-pressure homogenization process. Potential cytotoxic effects were evaluated by the MTT assay using two human intestinal cell models (Caco-2 and HT29-MTX-E12). Since in humans the digestion process may modify the physicochemical properties of NMs, potentially influencing biological outcomes, the CNFs were subjected to a harmonized in vitro digestion method before cytotoxicity testing. No cytotoxic effect was observed after 24h exposure to the undigested CNFs in the concentration-range tested (3.1 µg/mL-200 µg/mL), irrespectively of the cell line used. Similar results were obtained for the digested CNFs for concentrations up to 14.3 µg/mL. The observation that the in vitro digestion mixture was cytotoxic by itself for concentrations above 7.6 % (v/v) (i.e., equivalent to CNMs exposure > 14.3 µg/mL) impaired cytotoxicity assessment at higher CNFs concentrations. Complementary cytotoxicity assays and future optimization of the in vitro digestion procedure to reduce its toxicity are underway, to refine the assessment of CNFs cytotoxicity, particularly after digestion. Furthermore, genotoxicity studies will increase the knowledge on the cellular effects of CNMs in the human intestine, contributing to the safety assessment of CNMs early in its development stage, towards sustainable innovation of nanomaterials, thereby protecting human health.pt_PT
dc.description.sponsorshipFCT/MCTES through national funds (PTDC/SAU-PUB/29481/2017; PTDC/SAUPUB/ 32587/2017) and (UIDB/00009/2020; UIDP/00009/2020); FCT/MCTES PhD studentship 2020.07168.BD was attributed to NVpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/7848
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationPTDC/SAUPUB/ 32587/2017pt_PT
dc.relationCentre for Toxicogenomics and Human Health
dc.relationCentre for Toxicogenomics and Human Health
dc.subjectNanomaterialspt_PT
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectCellulose Nanomaterialspt_PT
dc.subjectNanotoxicologypt_PT
dc.subjectIn vitro Simulated Digestionpt_PT
dc.subjectIntestinal Epithelial Cellspt_PT
dc.subjectCytotoxicitypt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleCytotoxicity Assessment of Novel Cellulose Nanomaterials on Intestinal Cellspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-PUB%2F29481%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00009%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00009%2F2020/PT
oaire.citation.conferencePlace(online)pt_PT
oaire.citation.title4th International Conference on Food Contaminants (ICFC2021): Challenges on Early-Life Exposure, 27-28 set 2021 (online)pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameSilva
person.familyNameLouro
person.givenNameMaria Joao
person.givenNameHenriqueta
person.identifier157627
person.identifier.ciencia-id7710-643D-97A3
person.identifier.ciencia-id721D-2BB1-7DB1
person.identifier.orcid0000-0002-6060-0716
person.identifier.orcid0000-0001-9744-7332
person.identifier.scopus-author-id55944437600
person.identifier.scopus-author-id6507971479
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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relation.isAuthorOfPublication.latestForDiscovery2361a951-8b9a-4b90-92d6-f6384003a242
relation.isProjectOfPublication10a38358-808e-4126-a323-58973bebbcce
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relation.isProjectOfPublication8836ec79-2300-487a-97ad-75e98cfabcfa
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