Publicação
Prediction of Antibiotic Resistance Genes in Cyanobacterial Strains by Whole Genome Sequencing
| dc.contributor.author | Balata, Duarte | |
| dc.contributor.author | Rosado, Tânia | |
| dc.contributor.author | Pina-Martins, Francisco | |
| dc.contributor.author | Manageiro, Vera | |
| dc.contributor.author | Menezes, Carina | |
| dc.contributor.author | Ferreira, Eugénia | |
| dc.contributor.author | Paulo, Octávio S. | |
| dc.contributor.author | Caniça, Manuela | |
| dc.contributor.author | Dias, Elsa | |
| dc.date.accessioned | 2026-02-06T15:24:17Z | |
| dc.date.available | 2026-02-06T15:24:17Z | |
| dc.date.issued | 2025-05-28 | |
| dc.description.abstract | Cyanobacteria are ubiquitous in freshwater environments, but their role in aquatic resistome remains unclear. In this work, we performed whole genome sequencing on 43 cyanobacterial strains isolated from Portuguese fresh/wastewaters. From 43 available non-axenic unicyanoabacterial cultures (containing only one cyanobacterial strain and their co-occurring bacteria), it was possible to recover 41 cyanobacterial genomes from the genomic assemblies using a genome binning software, 26 of which were classified as high-quality based on completeness, contamination, N50 and contig number thresholds. By using the comprehensive antibiotic resistance database (CARD) on the assembled samples, we detected four antibiotic resistance gene (ARG) variants, conferring resistance in pathogenic bacteria to tetracyclines, fluoroquinolones (-type) and macrolides (-type, -type and -type). Among these, -type was the most prevalent gene, found across 11 cyanobacterial genomes from the Nostocales order. presented the highest variety of close ARG matches, with hits for the macrolide resistance genes -type, -type and type. An analysis of the genomic assemblies also revealed an additional 12 ARGs in bacteria from the phyla Firmicutes, Proteobacteria and Bacteroidetes, present in the cyanobacterial cultures, foreseeing the horizontal gene transfer of ARGs with cyanobacteria. Additionally, more than 200 partial ARGs were detected on each recovered cyanobacterial genome, allowing for future studies of antibiotic resistance genotype/phenotype in cyanobacteria. These findings highlight the importance of further efforts to understand the role of cyanobacteria on the aquatic resistome from a One Health perspective. | eng |
| dc.description.sponsorship | This work was performed in the scope of the project “ExplorAR—Exploring the Aquatic Resistome” (PTDC/BIA-BMA/31451/2017), financed by the Portuguese Foundation for Science and Technology (FCT/MCTES). This support also included the fellowship attributed to Duarte Balata and the research contract of Tânia Rosado. This work was also supported by funding from FCT/MCTES, attributed to CECA (UIDB/00211/2020). | |
| dc.identifier.citation | Microorganisms. 2025 May 28;13(6):1252. doi: 10.3390/microorganisms13061252 | |
| dc.identifier.doi | 10.3390/microorganisms13061252 | |
| dc.identifier.eissn | 2076-2607 | |
| dc.identifier.pmid | 40572139 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/10837 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | MDPI | |
| dc.relation | Exploring the aquatic resistome. | |
| dc.relation | Center for the Study of Animal Science | |
| dc.relation.hasversion | https://www.mdpi.com/2076-2607/13/6/1252 | |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject | Antibiotic Resistance | |
| dc.subject | Aquatic Resistome | |
| dc.subject | Bioinformatics Pipeline | |
| dc.subject | Cyanobacteria | |
| dc.subject | Freshwater Bacteria | |
| dc.subject | Genome | |
| dc.subject | Resistência aos Antimicrobianos | |
| dc.title | Prediction of Antibiotic Resistance Genes in Cyanobacterial Strains by Whole Genome Sequencing | eng |
| dc.type | journal article | |
| dcterms.references | https://www.mdpi.com/article/10.3390/microorganisms13061252/s1 | |
| dcterms.references | The raw sequence reads used for the assembly were deposited in the National Center for Biotechnology Information (NCBI) Sequence Read Archive under accession numbers SAMN34233939 to SAMN34233979 (Bioproject ref. PRJNA956929). | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Exploring the aquatic resistome. | |
| oaire.awardTitle | Center for the Study of Animal Science | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-BMA%2F31451%2F2017/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00211%2F2020/PT | |
| oaire.citation.issue | 6 | |
| oaire.citation.startPage | 1252 | |
| oaire.citation.title | Microorganisms | |
| oaire.citation.volume | 13 | |
| oaire.fundingStream | 3599-PPCDT | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
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| relation.isProjectOfPublication | 69b75eb9-6f25-4ad8-98db-6cc7e9bcdcc7 | |
| relation.isProjectOfPublication.latestForDiscovery | 17bea914-3ac3-470b-bc8f-115cd5086a2b |