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Hazard assessment of benchmark metallic nanomaterials in alveolar epithelial cells

2017-09-10Conference object Restricted access
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dc.contributor.authorSaruga, Andreia
dc.contributor.authorLouro, Henriqueta
dc.contributor.authorPinhão, Mariana
dc.contributor.authorSilva, Maria João
dc.date.accessioned2018-02-20T16:32:20Z
dc.date.available2018-02-20T16:32:20Z
dc.date.issued2017-09-10
dc.descriptionAbstract publicado em: Toxicology Letters. 2017;280 (Suppl 1):S180–S190. Disponível em: http://dx.doi.org/10.1016/j.toxlet.2017.07.523pt_PT
dc.description.abstractThe fast development of nanotechnology has led to the manufacturing of a wide array of nanomaterials (NMs). Despite the number of studies addressing NMs toxicity, uncertainties about their safety remain, representing a challenge to regulatory authorities. This work intended to assess the toxicity of metallic NMs in alveolar epithelial cells and to relate the effects with their physicochemical properties. Benchmark NMs (JRC) - CeO2 (NM-212), TiO2 (NM-100) and BaSO4 (NM-220) - were dispersed and their properties in the culture medium were evaluated by DLS. A549 cells were exposed to each NM for cytotoxicity (MTT and plating efficiency assays) and genotoxicity (comet and cytokinesis-blocked micronucleus, CBMN, assays) assessment. A homogeneous dispersion that remained stable in culture medium was achieved for all NMs. The CeO2NM was the only one that decreased cells’ proliferative capacity after 8 days exposure. As to the genotoxicity, the TiO2NM significantly increased the level of DNA damage following 3h and 24h exposure, whereas the CeO2NM caused only a slight increase in DNA damage at 3h exposure. None of the NMs tested positive by the CBMN assay. BaSO4NM was neither cytotoxic nor genotoxic. In conclusion, this study contributed to the hazard assessment of different benchmark metallic NMs, disclosing diverse biological effects that will be interpreted considering the inherent physicochemical properties. The identification of key features and pathways that drive NMs’ toxicity is paramount to allow prediction of their adverse effects, avoiding the huge task of testing every new NM.pt_PT
dc.description.sponsorshipProject NANoREG (GA 310584), ToxOmics (UID/BIM/00009/2013) and INSA (2013DGH860).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/5004
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationA common European approach to the regulatory testing of nanomaterials
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectMetallic Nanomaterialspt_PT
dc.subjectNanosafetypt_PT
dc.subjectHazard Assessmentpt_PT
dc.subjectRespiratory Cellspt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleHazard assessment of benchmark metallic nanomaterials in alveolar epithelial cellspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleA common European approach to the regulatory testing of nanomaterials
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/310584/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIM%2F00009%2F2013/PT
oaire.citation.conferencePlaceBratislava, Eslováquiapt_PT
oaire.citation.title53rd Congress of the European Societies of Toxicology (EuroTox), 10-13th September 2017pt_PT
oaire.fundingStreamFP7
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isProjectOfPublicationf9fe5c15-606f-4958-ac4b-8109981d34c7
relation.isProjectOfPublicatione9cc9728-4f09-4e3a-b30d-53d4429986fb
relation.isProjectOfPublication.latestForDiscoveryf9fe5c15-606f-4958-ac4b-8109981d34c7

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