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In vitro cytotoxicity of superparamagnetic iron oxide nanoparticles on neuronal and glial cells. Evaluation of nanoparticle interference with viability tests

2015-07-26Journal article Restricted access
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dc.contributor.authorCosta, Carla
dc.contributor.authorBrandão, Fátima
dc.contributor.authorBessa, Maria João
dc.contributor.authorCosta, Solange
dc.contributor.authorValdiglesias, Vanessa
dc.contributor.authorKiliç, Gözde
dc.contributor.authorFernández-Bertólez, Natalia
dc.contributor.authorQuaresma, Pedro
dc.contributor.authorPereira, Eulália
dc.contributor.authorPásaro, Eduardo
dc.contributor.authorLaffon, Blanca
dc.contributor.authorTeixeira, João Paulo
dc.date.accessioned2016-02-16T17:48:10Z
dc.date.available2016-02-16T17:48:10Z
dc.date.issued2015-07-26
dc.description.abstractSuperparamagnetic iron oxide nanoparticles (ION) have attracted great interest for use in several biomedical fields. In general, they are considered biocompatible, but little is known of their effects on the human nervous system. The main objective of this work was to evaluate the cytotoxicity of two ION (magnetite), coated with silica and oleic acid, previously determining the possible interference of the ION with the methodological procedures to assure the reliability of the results obtained. Human neuroblastoma SHSY5Y and glioblastoma A172 cells were exposed to different concentrations of ION (5-300 µg ml(-1) ), prepared in complete and serum-free cell culture medium for three exposure times (3, 6 and 24 h). Cytotoxicity was evaluated by means of the MTT, neutral red uptake and alamar blue assays. Characterization of the main physical-chemical properties of the ION tested was also performed. Results demonstrated that both ION could significantly alter absorbance readings. To reduce these interferences, protocols were modified by introducing additional washing steps and cell-free systems. Significant decreases in cell viability were observed for both cell lines in specific conditions by all assays. In general, oleic acid-coated ION were less cytotoxic than silica-coated ION; besides, a serum-protective effect was observed for both ION studied and cell lines. These results contribute to increase the knowledge of the potential harmful effects of ION on the human nervous system. Understanding these effects is essential to establish satisfactory regulatory policies on the safe use of magnetite nanoparticles in biomedical applications.pt_PT
dc.description.sponsorshipThis work was funded by Xunta de Galicia (EM 2012/079) and by TD1204 MODENA COST Action. G.K. was supported by a fellowship from the University of A Coruña.pt_PT
dc.identifier.citationJ Appl Toxicol. 2016 Mar;36(3):361-72. doi: 10.1002/jat.3213. Epub 2015 Jul 26.pt_PT
dc.identifier.doi10.1002/jat.3213pt_PT
dc.identifier.issn0260-437X
dc.identifier.urihttp://hdl.handle.net/10400.18/3350
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherJohn Wiley & Sons, Ltd.pt_PT
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1002/jat.3213/fullpt_PT
dc.subjectIron Oxide Nanoparticlespt_PT
dc.subjectToxicologypt_PT
dc.subjectCytotoxicitypt_PT
dc.subjectGlioblastoma Cellspt_PT
dc.subjectMagnetitept_PT
dc.subjectNeuroblastoma Cellspt_PT
dc.subjectOleic Acid-coated Iron Oxide Nanoparticlespt_PT
dc.subjectSilica-coated Iron Oxide Nanoparticlespt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleIn vitro cytotoxicity of superparamagnetic iron oxide nanoparticles on neuronal and glial cells. Evaluation of nanoparticle interference with viability testspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage372pt_PT
oaire.citation.startPage361pt_PT
oaire.citation.titleJournal of Applied Toxicologypt_PT
oaire.citation.volume36(3)pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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