Evaluating the genotoxicity of cellulose nanofibrils in a co-culture of human lung epithelial cells and monocyte-derived macrophages

Ventura, Célia; Lourenço, Ana Filipa; Sousa-Uva, António; Ferreira, Paulo J.T.; Silva, Maria João

Publication

Evaluating the genotoxicity of cellulose nanofibrils in a co-culture of human lung epithelial cells and monocyte-derived macrophages

2018-07Journal article

dc.contributor.author	Ventura, Célia
dc.contributor.author	Lourenço, Ana Filipa
dc.contributor.author	Sousa-Uva, António
dc.contributor.author	Ferreira, Paulo J.T.
dc.contributor.author	Silva, Maria João
dc.date.accessioned	2019-02-20T10:37:03Z
dc.date.available	2019-02-20T10:37:03Z
dc.date.issued	2018-07
dc.description.abstract	Cellulose nanofibrils (CNF) are manufactured nanofibres that hold impressive expectations in forest, food, pharmaceutical, and biomedical industries. CNF production and applications are leading to an increased human exposure and thereby it is of utmost importance to assess its safety to health. In this study, we screened the cytotoxic, immunotoxic and genotoxic eﬀects of a CNF produced by TEMPO-mediated oxidation of an industrial bleached Eucalyptus globulus kraft pulp on a co-culture of lung epithelial alveolar (A549) cells and monocyte-derived macrophages (THP-1 cells). The results indicated that low CNF concentrations can stimulate A549 cells proliferation, whereas higher concentrations are moderately toxic. Moreover, no proinflammatory cytokine IL-1β was detected in the co-culture medium suggesting no immunotoxicity. Although CNF treatment did not induce sizable levels of DNA damage in A549 cells, it leaded to micronuclei formation at 1.5 and 3 μg/cm2. These findings suggest that this type of CNF is genotoxic through aneugenic or clastogenic mechanisms. Noteworthy, cell overgrowth and genotoxicity, which are events relevant for cell malignant transformation, were observed at low CNF concentration levels, which are more realistic and relevant for human exposure, e.g., in occupational settings.	pt_PT
dc.description.sponsorship	This research was co-funded through UID/BIM/00009/2013, Centre for Toxicogenomics and Human Health (ToxOmics), and SFRH/BDE/ 108095/2015, from the Foundation for Science and Technology, Portugal	pt_PT
dc.description.version	info:eu-repo/semantics/publishedVersion	pt_PT
dc.identifier.citation	Toxicol Lett. 2018 Jul;291:173-183. doi: 10.1016/j.toxlet.2018.04.013. Epub 2018 Apr 18.	pt_PT
dc.identifier.doi	doi.org/10.1016/j.toxlet.2018.04.013	pt_PT
dc.identifier.issn	0378-4274
dc.identifier.uri	http://hdl.handle.net/10400.18/5909
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	Elsevier/EUROTOX	pt_PT
dc.relation.publisherversion	https://www.sciencedirect.com/science/article/pii/S0378427418301504?via%3Dihub	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	pt_PT
dc.subject	Cellulose Nanofibrils	pt_PT
dc.subject	Safety Assessment	pt_PT
dc.subject	Immunotoxicity	pt_PT
dc.subject	Comet Assay	pt_PT
dc.subject	Micronucleus Assay	pt_PT
dc.subject	Genotoxicidade Ambiental	pt_PT
dc.title	Evaluating the genotoxicity of cellulose nanofibrils in a co-culture of human lung epithelial cells and monocyte-derived macrophages	pt_PT
dc.type	journal article
dspace.entity.type	Publication
oaire.awardURI	info:eu-repo/grantAgreement/FCT/5876/UID%2FBIM%2F00009%2F2013/PT
oaire.citation.endPage	183	pt_PT
oaire.citation.startPage	173	pt_PT
oaire.citation.title	Toxicology Letters	pt_PT
oaire.citation.volume	291	pt_PT
oaire.fundingStream	5876
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.name	Fundação para a Ciência e a Tecnologia
rcaap.embargofct	Política editorial	pt_PT
rcaap.rights	embargoedAccess	pt_PT
rcaap.type	article	pt_PT
relation.isProjectOfPublication	e9cc9728-4f09-4e3a-b30d-53d4429986fb
relation.isProjectOfPublication.latestForDiscovery	e9cc9728-4f09-4e3a-b30d-53d4429986fb