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Diversity of extended-spectrum and plasmid-mediated AmpC β-lactamases in Enterobacteriaceae isolates from Portuguese health care facilities

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dc.contributor.authorJones-Dias, Daniela
dc.contributor.authorManageiro, Vera
dc.contributor.authorFerreira, Eugénia
dc.contributor.authorLouro, Deolinda
dc.contributor.authorAntibiotic Resistance Surveillance Program in Portugal (ARSIP) participants
dc.contributor.authorCaniça, Manuela
dc.date.accessioned2015-02-25T17:05:47Z
dc.date.available2015-02-25T17:05:47Z
dc.date.issued2014
dc.description.abstractA group of 124 Enterobacteriaceae isolates resistant to third generation cephalosporins, and collected in distinct health care facilities of different Portuguese regions was analysed. The great majority of the isolates were also resistant to fourth generation cephalosporins (83.9%), monobactam (96%), amoxicillin plus clavulanic acid (85.5%), and piperacillin plus tazobactam (66.9%). Overall, 84.7% (105/124) were multidrug resistant. Molecular methods enabled us to identify 86.3% (107/124) extended-spectrum β-lactamases (ESBL) producers, revealing a diversity of class A β-lactamases from different families, like TEM (TEM-1, TEM-10, TEM-24, and TEM-52), SHV (SHV-1, SHV-12, and SHV-28), CTX-M (CTX-M-1, CTX-M-9, CTX-M-14, CTX-M-15, and CTXM-32), and GES (GES-1). We have also detected class C enzymes like plasmid-mediated AmpC β-lactamases (PMAβs, DHA-1, and CMY-2) and chromosomal AmpCs in Enterobacter and Citrobacter spp. The PMAβ genetic context mapping suggests association with mobile elements, plasmid importation and the potential emergence of these β-lactamases. The most prevalent β-lactamase detected was CTX-M-15 (66.1%) and in 41.1% of the isolates it was associated with TEM-, OXA-type β-lactamases and Aac(6)᾿Ib-cr, which might indicate that the respective genotype has settled in our country. Indeed, CTX-M-15 was distributed amongst distinct clinical settings of several health care facilities (93.5%) from various regions. We provide evidence of a concerning clinical situation that includes vast occurrence of ESBLs, the settling of CTX-M β-lactamases, and the report of plasmidic and chromosomal AmpC in Portugal.por
dc.description.sponsorshipD. Jones-Dias was supported by grant BRJ/02/DG/2009 from NIH Dr. Ricardo Jorge, Lisbon, Portugal. V. Manageiro was supported by grant SFRH/BD/32578/2006 from Fundação para a Ciência e a Tecnologia, Lisbon, Portugal.por
dc.identifier.citationJ Microbiol. 2014 Jun;52(6):496-503. doi: 10.1007/s12275-014-3420-x. Epub 2014 May 29por
dc.identifier.doi10.1007/s12275-014-3420-x
dc.identifier.issn1225-8873
dc.identifier.urihttp://hdl.handle.net/10400.18/2957
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringer Verlag/ Microbiological Society of Koreapor
dc.relation.publisherversionhttp://link.springer.com/article/10.1007%2Fs12275-014-3420-xpor
dc.subjectResistência aos Antibióticospor
dc.subjectDiversitypor
dc.subjectESBLpor
dc.subjectPMABpor
dc.subjectHealth Care Facilitiespor
dc.subjectPortugal
dc.titleDiversity of extended-spectrum and plasmid-mediated AmpC β-lactamases in Enterobacteriaceae isolates from Portuguese health care facilitiespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage503por
oaire.citation.startPage496por
oaire.citation.titleJournal of Microbiologypor
oaire.citation.volume52(6)por
rcaap.rightsembargoedAccesspor
rcaap.typearticlepor

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