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Drinking water contaminants: toxicity of halogenated polycyclic aromatic hydrocarbons

2015-04-12Conference object Open access
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dc.contributor.authorJosé, S.S.
dc.contributor.authorPinto, M.
dc.contributor.authorAntunes, A.M.M.
dc.contributor.authorLouro, H.
dc.contributor.authorJordão, Luísa
dc.contributor.authorSilva, M.J.
dc.contributor.authorCardoso, A.S.
dc.date.accessioned2015-10-12T15:41:50Z
dc.date.available2015-10-12T15:41:50Z
dc.date.issued2015-04-12
dc.description.abstractFood may be contaminated with polycyclic aromatic hydrocarbons (PAHs) in the process of smoking or heating. These contaminants or their derivatives can also be present in drinking water when raw water contacts with discharges of untreated industrial/waste water effluents, forest fires or by solubilisation of organic material from contaminated soils. A few studies have shown that water disinfection can lead to halogenated derivatives of PAHs (HPAHs) as chlorinated and brominated derivatives, and there are evidences that these compounds may have greater mutagenicity than the parent PAHs. In this study the cytotoxic and genotoxic effects of chlorinated/brominated derivatives of pyrene (Pyr) and benzo[a]anthracene (BaA), 1-ClPyr, 1-BrPyr and 7-ClBaA, which can be formed as water disinfection by-products, were studied in HepG2 cells to assess their potential hazard to human health. The formation of 1-ClPyr, 1-BrPyr and 7-ClBaA under aqueous disinfection conditions in waters contaminated with Pyr and BaA, was confirmed with an optimized gas chromatography method. Cells exposed (24h) to several concentrations of BaA and 7-ClBaA (1 to 200μM) displayed a dose-related and significant increase of cytotoxicity (neutral red assay) with IC50 values of 3.37 and 12.63µM respectively. For Pyr, 1-ClPyr and 1-BrPyr (10 to 200μM), a lower but significant dose-related cytotoxicity was observed. At non-cytotoxic concentrations (10 and 15µM), 7-ClBaA was able to induce a significantly higher level of oxidative DNA damage in HepG2 cells than its parent compound, as assessed by the FPG-modified comet assay. Under these conditions neither Pyr nor its derivatives were genotoxic. In conclusion, the disinfection process may give rise to genotoxic HPAHs with potential impact on human health and it should be performed in raw waters with minimal content of total organic carbon. In real conditions, humans may be exposed to a mixture of these organic compounds and thus their combined toxic effects should be further evaluated.pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/3194
dc.language.isoengpt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.subjectÁgua e Solopt_PT
dc.subjectPolycyclic Aromatic Hydrocarbonspt_PT
dc.subjectPAHspt_PT
dc.subjectHPAHspt_PT
dc.titleDrinking water contaminants: toxicity of halogenated polycyclic aromatic hydrocarbonspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceLisboa, Portugalpt_PT
oaire.citation.titleInternational Conference on Food Contaminants: challenges in chemical mixtures (ICFC2015), 13-14th April 2015pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT

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