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Unravelling the Potential Cytotoxic Effects of Metal Oxide Nanoparticles and Metal(Loid) Mixtures on A549 Human Cell Line

2020-03-02Journal article Open access
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dc.contributor.authorRosário, Fernanda
dc.contributor.authorBessa, Maria João
dc.contributor.authorBrandão, Fátima
dc.contributor.authorCosta, Carla
dc.contributor.authorLopes, Cláudia B.
dc.contributor.authorEstrada, Ana C.
dc.contributor.authorTavares, Daniela S.
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorReis, Ana Teresa
dc.date.accessioned2021-03-03T15:53:13Z
dc.date.available2021-03-03T15:53:13Z
dc.date.issued2020-03-02
dc.description.abstractHumans are typically exposed to environmental contaminants' mixtures that result in different toxicity than exposure to the individual counterparts. Yet, the toxicology of chemical mixtures has been overlooked. This work aims at assessing and comparing viability and cell cycle of A549 cells after exposure to single and binary mixtures of: titanium dioxide nanoparticles (TiO2NP) 0.75-75 mg/L; cerium oxide nanoparticles (CeO2NP) 0.0.75-10 μg/L; arsenic (As) 0.75-2.5 mg/L; and mercury (Hg) 5-100 mg/L. Viability was assessed through water-soluble tetrazolium (WST-1) and thiazolyl blue tetrazolium bromide (MTT) (24 h exposure) and clonogenic (seven-day exposure) assays. Cell cycle alterations were explored by flow cytometry. Viability was affected in a dose- and time-dependent manner. Prolonged exposure caused inhibition of cell proliferation even at low concentrations. Cell-cycle progression was affected by TiO2NP 75 mg/L, and As 0.75 and 2.5 μg/L, increasing the cell proportion at G0/G1 phase. Combined exposure of TiO2NP or CeO2NP mitigated As adverse effects, increasing the cell surviving factor, but cell cycle alterations were still observed. Only CeO2NP co-exposure reduced Hg toxicity, translated in a decrease of cells in Sub-G1. Toxicity was diminished for both NPs co-exposure compared to its toxicity alone, but a marked toxicity for the highest concentrations was observed for longer exposures. These findings prove that joint toxicity of contaminants must not be disregarded.pt_PT
dc.description.sponsorshipProject NanoLegaTox (PTDC/SAU-PUB/29651/2017) cofinanced by COMPETE 2020, Portugal 2020 and European Union, through FEDER.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNanomaterials (Basel). 2020 Mar 2;10(3):447. doi: 10.3390/nano10030447.pt_PT
dc.identifier.doi10.3390/nano10030447pt_PT
dc.identifier.issn2079-4991
dc.identifier.urihttp://hdl.handle.net/10400.18/7295
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2079-4991/10/3/447pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCytotoxicitypt_PT
dc.subjectCo-exposurept_PT
dc.subjectMixturespt_PT
dc.subjectA549pt_PT
dc.subjectArsenicpt_PT
dc.subjectMercurypt_PT
dc.subjectTitanium Dioxide Nanoparticlespt_PT
dc.subjectCerium Oxide Nanoparticlespt_PT
dc.subjectClonogenic Assaypt_PT
dc.subjectCell-cyclept_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleUnravelling the Potential Cytotoxic Effects of Metal Oxide Nanoparticles and Metal(Loid) Mixtures on A549 Human Cell Linept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FSAU-PUB%2F29651%2F2017/PT
oaire.citation.issue3pt_PT
oaire.citation.startPage447pt_PT
oaire.citation.titleNanomaterials (Basel)pt_PT
oaire.citation.volume10pt_PT
oaire.fundingStream9471 - RIDTI
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication9f23e866-c585-4ebc-af90-ee03cf2d2655
relation.isProjectOfPublication.latestForDiscovery9f23e866-c585-4ebc-af90-ee03cf2d2655

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