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Analysis of the In Vitro Toxicity of Nanocelluloses in Human Lung Cells as Compared to Multi-Walled Carbon Nanotubes

2022-04-22Journal article Open access
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dc.contributor.authorPinto, Fátima
dc.contributor.authorLourenço, Ana Filipa
dc.contributor.authorPedrosa, Jorge F.S.
dc.contributor.authorGonçalves, Lídia
dc.contributor.authorVentura, Célia
dc.contributor.authorVital, Nádia
dc.contributor.authorBettencourt, Ana
dc.contributor.authorFernandes, Susete N.
dc.contributor.authorda Rosa, Rafaela R.
dc.contributor.authorGodinho, Maria Helena
dc.contributor.authorLouro, Henriqueta
dc.contributor.authorFerreira, Paulo J.T.
dc.contributor.authorSilva, Maria João
dc.date.accessioned2022-11-24T12:19:40Z
dc.date.available2022-11-24T12:19:40Z
dc.date.issued2022-04-22
dc.descriptionThis article belongs to the Special Issue Cytotoxicity and Genotoxicity Assessment in Nanomaterials.pt_PT
dc.description.abstractCellulose micro/nanomaterials (CMNM), comprising cellulose microfibrils (CMF), nanofibrils (CNF), and nanocrystals (CNC), are being recognized as promising bio-nanomaterials due to their natural and renewable source, attractive properties, and potential for applications with industrial and economical value. Thus, it is crucial to investigate their potential toxicity before starting their production at a larger scale. The present study aimed at evaluating the cell internalization and in vitro cytotoxicity and genotoxicity of CMNM as compared to two multi-walled carbon nanotubes (MWCNT), NM-401 and NM-402, in A549 cells. The exposure to all studied NM, with the exception of CNC, resulted in evident cellular uptake, as analyzed by transmission electron microscopy. However, none of the CMNM induced cytotoxic effects, in contrast to the cytotoxicity observed for the MWCNT. Furthermore, no genotoxicity was observed for CNF, CNC, and NM-402 (cytokinesis-block micronucleus assay), while CMF and NM-401 were able to significantly raise micronucleus frequency. Only NM-402 was able to induce ROS formation, although it did not induce micronuclei. Thus, it is unlikely that the observed CMF and NM-401 genotoxicity is mediated by oxidative DNA damage. More studies targeting other genotoxicity endpoints and cellular and molecular events are underway to allow for a more comprehensive safety assessment of these nanocelluloses.pt_PT
dc.description.sponsorshipThis research received funding from the Portuguese Foundation for Science and Technology (FCT/MCTES), through national funds (PIDDAC) under the project ToxApp4NanoCELFI (PTDC/SAUPUB/32587/2017). The following financial grants are also acknowledged: Projects UIDB/00009/2020 and UIDP/00009/2020 (ToxOmics); Projects UIDB/04138/2020 and UIDP/04138/2020 (iMed.Ulisboa) and L. Gonçalves Principal Researcher grant (CEECIND/03143/2017); FEDER and POR Lisboa2020 through the COMPETE 2020 POCI and PORL; POCI-01-0145-FEDER-007688 (UIDB/50025/2020- 2023); NanoCell2SEC (PTDC/CTM-REF/30529/2017); CIEPQPF (UIDB/00102/2020); European Topology Interdisciplinary Action (EUTOPIA CA17139); Inpactus Project—Innovative Products and Technologies from Eucalyptus (Project Nº 21874, funded by Portugal 2020 through ERDF in the frame of COMPETE 2020 Nº246/AXIS II/2017). Nádia Vital holds an FCT/MCTES PhD Scholarship grant (2020.07168.BD)pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNanomaterials (Basel). 2022 Apr 22;12(9):1432. doi: 10.3390/nano12091432pt_PT
dc.identifier.doi10.3390/nano12091432pt_PT
dc.identifier.issn2079-4991
dc.identifier.urihttp://hdl.handle.net/10400.18/8332
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationA predictive toxicology approach to characterize potential respiratory effects of functionalized nanocellulose fibres in a co-culture system
dc.relationCentre for Toxicogenomics and Human Health
dc.relationResearch Institute for Medicines
dc.relationResearch Institute for Medicines
dc.relationNot Available
dc.relationInstitute of Nanostructures, Nanomodelling and Nanofabrication
dc.relationChemical Process Engineering and Forest Products Research Centre
dc.relationToxicological profile of cellulose nanomaterials in human gastrointestinal cells
dc.relation.publisherversionhttps://www.mdpi.com/2079-4991/12/9/1432pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectMicro/nanocellulosespt_PT
dc.subjectIn vitro Cytotoxicitypt_PT
dc.subjectGenotoxicitypt_PT
dc.subjectMicronucleus Assaypt_PT
dc.subjectCell Uptakept_PT
dc.subjectReactive Oxygen Speciespt_PT
dc.subjectNanocellulosept_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleAnalysis of the In Vitro Toxicity of Nanocelluloses in Human Lung Cells as Compared to Multi-Walled Carbon Nanotubespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleA predictive toxicology approach to characterize potential respiratory effects of functionalized nanocellulose fibres in a co-culture system
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleNot Available
oaire.awardTitleInstitute of Nanostructures, Nanomodelling and Nanofabrication
oaire.awardTitleChemical Process Engineering and Forest Products Research Centre
oaire.awardTitleToxicological profile of cellulose nanomaterials in human gastrointestinal cells
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-PUB%2F32587%2F2017/PT
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F03143%2F2017%2FCP1476%2FCT0005/PT
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00102%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//2020.07168.BD/PT
oaire.citation.issue9pt_PT
oaire.citation.startPage1432pt_PT
oaire.citation.titleNanomaterialspt_PT
oaire.citation.volume12pt_PT
oaire.fundingStream3599-PPCDT
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oaire.fundingStream9471 - RIDTI
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person.familyNameLouro
person.familyNameSilva
person.givenNameHenriqueta
person.givenNameMaria Joao
person.identifier157627
person.identifier.ciencia-id721D-2BB1-7DB1
person.identifier.ciencia-id7710-643D-97A3
person.identifier.orcid0000-0001-9744-7332
person.identifier.orcid0000-0002-6060-0716
person.identifier.scopus-author-id6507971479
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rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
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