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Development and testing of microsatellite loci for the study of population genetics of Ixodes ricinus Linnaeus, 1758 and Ixodes inopinatus Estrada-Peña, Nava & Petney, 2014 (Acari: Ixodidae) in the western Mediterranean region

2023-03Journal article Restricted access
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dc.contributor.authorVelez, Rita
dc.contributor.authorDe Meeûs, Thierry
dc.contributor.authorBeati, Lorenza
dc.contributor.authorYounsi, Hend
dc.contributor.authorZhioua, Elyes
dc.contributor.authorAntunes, Sandra
dc.contributor.authorDomingos, Ana
dc.contributor.authorAtaíde Sampaio, Daniel
dc.contributor.authorCarpinteiro, Dina
dc.contributor.authorMoerbeck, Leonardo
dc.contributor.authorEstrada-Peña, Agustin
dc.contributor.authorSantos-Silva, Maria Margarida
dc.contributor.authorSantos, Ana Sofia
dc.date.accessioned2024-01-29T14:18:09Z
dc.date.available2024-01-29T14:18:09Z
dc.date.issued2023-03
dc.description.abstractIxodes ricinus is an important vector of several human and veterinary infectious agents. Its wide geographical distribution and permissive feeding behaviour have prompted earlier studies on its population genetics. Results were, nevertheless, not conclusive. Furthermore, no research has fully focused on the south-western distribution range of I. ricinus, where exchanges between European and North African populations are more likely to occur. The presence of an additional species, Ixodes inopinatus, in the area further confuses the topic, as the two species are hard to differentiate morphologically. The present work describes the testing of microsatellite markers previously described for I. ricinus using Portuguese and Tunisian tick populations of both species. In addition, new microsatellite loci were developed to complement the available marker toolbox. Loci showed different amplification successes across subpopulations, with Tunisian DNA less readily amplified. Altogether, 15 loci were considered suitable for genetic analyses of Portuguese subpopulations, 10 for Tunisian samples, and seven, common to both populations, were considered to be informative at the inter-continental level. A preliminary analysis of both datasets revealed two isolated populations, which can correspond to two different species. Furthermore, Tunisian specimens identified by sequencing of 16S rDNA as having I. ricinus or I. inopinatus sequence profiles all clustered together in one single population using the proposed microsatellites. This confirms that taxonomic decisions based only on 16S rRNA gene sequencing can be misleading. The application of the proposed set of microsatellite markers to a larger sample, representative of the south-western Ixodes’ distribution range, will be crucial to clarify the distribution of both species.pt_PT
dc.description.sponsorshipThis study was supported by FCT (Project TickGenoMi, reference PTDC/SAU-PAR/28947/2017).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAcarologia. 2023 Mar;63(2):356-372. doi:10.24349/bvem-4h49pt_PT
dc.identifier.doi10.24349/bvem-4h49pt_PT
dc.identifier.eissn2107-7207
dc.identifier.issn0044-586X
dc.identifier.urihttp://hdl.handle.net/10400.18/8987
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAcarologiapt_PT
dc.relationThe Ixodes ricinus group of ticks in the western Mediterranean region and north Africa: new insights in to their population genetics and microbiome fauna.
dc.relation.publisherversionhttps://www1.montpellier.inrae.fr/CBGP/acarologia/article.php?id=4596pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectIxodes ricinuspt_PT
dc.subjectIxodes Inopinatuspt_PT
dc.subjectMediterranean Regionpt_PT
dc.subjectMicrosatellitespt_PT
dc.subjectPopulation Geneticspt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleDevelopment and testing of microsatellite loci for the study of population genetics of Ixodes ricinus Linnaeus, 1758 and Ixodes inopinatus Estrada-Peña, Nava & Petney, 2014 (Acari: Ixodidae) in the western Mediterranean regionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleThe Ixodes ricinus group of ticks in the western Mediterranean region and north Africa: new insights in to their population genetics and microbiome fauna.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2017/PTDC%2FSAU-PAR%2F28947%2F2017/PT
oaire.citation.endPage372pt_PT
oaire.citation.issue63pt_PT
oaire.citation.startPage356pt_PT
oaire.citation.titleAcarologiapt_PT
oaire.citation.volume2pt_PT
oaire.fundingStreamConcurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2017
person.familyNamePerdigão Velez
person.familyNameSantos
person.givenNameMaria Rita
person.givenNameAna Sofia
person.identifier.ciencia-idF41F-4DFE-B957
person.identifier.ciencia-id8F1C-D6AD-0273
person.identifier.orcid0000-0003-2388-899X
person.identifier.orcid0000-0001-8824-228X
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione03f007e-242c-4a85-942a-9003e8bc75ce
relation.isAuthorOfPublication5658517a-3377-446d-8faa-4b9c4abaace9
relation.isAuthorOfPublication.latestForDiscoverye03f007e-242c-4a85-942a-9003e8bc75ce
relation.isProjectOfPublicationb4234abe-4028-4f77-9525-dcf8e4e7ea02
relation.isProjectOfPublication.latestForDiscoveryb4234abe-4028-4f77-9525-dcf8e4e7ea02

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