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Mitogenome diversity of Aedes (Stegomyia) albopictus: Detection of multiple introduction events in Portugal

2020-09-30Journal article Open access
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dc.contributor.authorZé-Zé, Líbia
dc.contributor.authorBorges, Vítor
dc.contributor.authorOsório, Hugo Costa
dc.contributor.authorMachado, Jorge
dc.contributor.authorGomes, João Paulo
dc.contributor.authorAlves, Maria João
dc.date.accessioned2021-03-31T16:50:13Z
dc.date.available2021-03-31T16:50:13Z
dc.date.issued2020-09-30
dc.description.abstractAedes albopictus, along with Ae. aegypti, are key arbovirus vectors that have been expanding their geographic range over the last decades. In 2017, Ae. albopictus was detected for the first time at two distinct locations in Portugal. In order to understand how the Ae. albopictus populations recently introduced in Portugal are genetically related and which is their likely route of invasion, we performed an integrative cytochrome C oxidase I gene (COI)- and mitogenome-based phylogeographic analysis of mosquitoes samples collected in Portugal in 2017 and 2018 in the context of the global Ae. albopictus diversity. COI-based analysis (31 partial sequences obtained from 83 mosquitoes) revealed five haplotypes (1 to 5), with haplotype 1 (which is widely distributed in temperate areas worldwide) being detected in both locations. Haplotypes 2 and 3 were exclusively found in Southern region (Algarve), while haplotype 4 and 5 were only detected in the North of Portugal (Penafiel, Oporto region). Subsequent high discriminatory analyses based on Ae. albopictus mitogenome (17 novel sequences) not only confirmed a high degree of genetic variability within and between populations at both geographic locations (compatible with the Ae. albopictus mosquito populations circulating in Europe), but also revealed two mitogenome mutational signatures not previously reported at worldwide level. While our results generally sustain the occurrence of multiple introduction events, fine mitogenome sequence inspection further indicates a possible Ae. albopictus migration within the country, from the Northern introduction locality to the Southern region. In summary, the observed scenario of high Ae. albopictus genetic diversity in Portugal, together with the detection of mosquitoes in successive years since 2017 in Algarve and Penafiel, points that both Ae. albopictus populations seem to be already locally established, as its presence has been reported for three consecutive years, raising the public health awareness for future mosquito-borne diseases outbreaks.pt_PT
dc.description.abstractAuthor summary: In 2017, Aedes albopictus was reported for the first time in Portugal at two distinct locations, in the premises of a tire company in Penafiel, in the North, and nearby a golf course in Algarve, a tourism destination in the southernmost country region. The geographical spread of this species is promoted by egg and larvae transport in aquatic trade goods, as tires and aquatic plants, and adult anthropophilic behavior that favors passive land transportation. In Portugal, especially in the Southern region, temperate climate conditions are adequate for adult mosquitoes to survive most of the year. In a way to understand the genetic variability of Ae. albopictus populations introduced in Portugal, we analyzed 31 cytochrome C oxidase I gene (COI) partial sequences and 17 mitogenome sequences, integrating them in the context of the global Ae. albopictus phylogeographic diversity (i.e., 183 COI and 26 mitogenome sequences previously reported at worldwide level). Although COI haplotype 1 predominated, four additional haplotypes (2 to 5) were detected in Portugal. Subsequent in-depth mitogenome analysis revealed considerable genetic diversity, including not only sequences relating to mitogenomes reported mainly from Italy, Japan and China, but also two novel mitogenome mutational signatures. Our study indicates that Ae. albopictus is locally established in Portugal and intra-country dispersal may have already happened, highlighting the challenges for vector surveillance and control programs aiming at restraining arbovirus disease burden in the future.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPLoS Negl Trop Dis. 2020 Sep 30;14(9):e0008657. doi: 10.1371/journal.pntd.0008657. eCollection 2020 Sep.pt_PT
dc.identifier.doi10.1371/journal.pntd.0008657pt_PT
dc.identifier.issn1935-2735
dc.identifier.urihttp://hdl.handle.net/10400.18/7618
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPublic Library of Sciencept_PT
dc.relation.publisherversionhttps://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0008657pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAedespt_PT
dc.subjectAnimalspt_PT
dc.subjectArbovirusespt_PT
dc.subjectElectron Transport Complex IVpt_PT
dc.subjectFemalept_PT
dc.subjectGenome, Mitochondrialpt_PT
dc.subjectHaplotypespt_PT
dc.subjectHigh-Throughput Nucleotide Sequencingpt_PT
dc.subjectMosquito Vectorspt_PT
dc.subjectPhylogeographypt_PT
dc.subjectPortugalpt_PT
dc.subjectSequence Analysis, DNApt_PT
dc.subjectGenetic Variationpt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleMitogenome diversity of Aedes (Stegomyia) albopictus: Detection of multiple introduction events in Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue9pt_PT
oaire.citation.startPagee0008657pt_PT
oaire.citation.titlePLOS Neglected Tropical Diseasespt_PT
oaire.citation.volume14pt_PT
rcaap.embargofctAcesso de acordo com página web do editor da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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