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Cyto-genotoxic effects of distinct micro/nanocelluloses in human osteoblastic and mice fibroblastic cells

2022-06-28Conference object Restricted access
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dc.contributor.authorVentura, Célia
dc.contributor.authorMarques, Catarina
dc.contributor.authorCadete, João
dc.contributor.authorVilar, Madalena
dc.contributor.authorPedrosa, J.F.S.
dc.contributor.authorPinto, P.
dc.contributor.authorRosa, R.R.
dc.contributor.authorFernandes, S.N.
dc.contributor.authorGodinho, M.H.
dc.contributor.authorFerreira, P.J.T.
dc.contributor.authorLouro, Henriqueta
dc.contributor.authorSilva, Maria João
dc.date.accessioned2022-11-28T11:03:44Z
dc.date.available2022-11-28T11:03:44Z
dc.date.issued2022-06-28
dc.description.abstractThe unique characteristics of micro/nanocelluloses (MNCs) have been boosting their use in a wide variety of industrial and biomedical applications. With the expected decrease of their production cost, it is likely to observe an even faster growing adoption of these materials in the coming years. With the increasing use of MNCs comes an increased likelihood of human exposure to these materials, raising concerns about potential human health effects. The number of studies on the potential adverse effects of MNCs is still scarce and sometimes inconclusive. The aim of the present study is to shed some light on the impact of human exposure to cellulose-based nanomaterials. For that, three MNCs were produced from bleached Eucalyptus kraft pulp using different pretreatments. A microfibrillated (CMF) and a nanofibrillated cellulose (CNF) were respectively obtained by enzymatic and TEMPO-mediated oxidation pretreatments followed by high-pressure homogenization. In addition, cellulose nanocrystals (CNC) were obtained by acid hydrolysis with diluted sulfuric acid. The potential cytotoxicity of the MNCs was evaluated in vitro in two mammalian cell lines (human MG-63 osteoblasts and mice V79 lung fibroblasts) by the MTT and the clonogenic assays, and the genotoxicity was assessed by the micronucleus assay. Cytotoxicity was observed by the clonogenic assay in V79 cells, particularly for CNC, but not by the MTT assay. CNF induced micronuclei in both cell lines and nucleoplasmic bridges in MG-63 cells. CMF and CNC induced micronuclei and nucleoplasmic bridges in MG-63 cells, but not in V79 cells. From the results, it was concluded that under the tested conditions all MNCs present cytotoxicity and genotoxicity, although at different concentration levels, which may be related to their physicochemical differences, to the availability for cell uptake, and to differences in the DNA damage response of the cell model.pt_PT
dc.description.sponsorshipThe work was funded by the Portuguese Foundation for Science and Technology (FCT/MCTES), through national funds (PIDDAC) under the project ToxApp4NanoCELFI (PTDC/SAU-PUB/32587/2017). The following funding is also acknowledged: ERDF and POR Lisboa2020 through the COMPETE 2020 POCI and PORL; POCI-01-0145- FEDER-007688 (UIDB/50025/2020-2023); NanoCell2SEC (PTDC/CTM-REF/30529/2017); ToxOmics (UIDP/00009/2020; UIDB/00009/2020); CIEPQPF (UIDB/00102/2020); European Topology Interdisciplinary Action (EUTOPIA CA17139) and Inpactus Project – innovative products and technologies from eucalyptus (Project N.º 21874 funded by Portugal 2020 through ERDF in the frame of COMPETE 2020 nº246/AXIS II/2017). Schemes created with BioRender.com.pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/8334
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationA predictive toxicology approach to characterize potential respiratory effects of functionalized nanocellulose fibres in a co-culture system
dc.relationInstitute of Nanostructures, Nanomodelling and Nanofabrication
dc.relationCentre for Toxicogenomics and Human Health
dc.relationCentre for Toxicogenomics and Human Health
dc.relationChemical Process Engineering and Forest Products Research Centre
dc.subjectNanocellulosespt_PT
dc.subjectMicrocellulosespt_PT
dc.subjectGenotoxicitypt_PT
dc.subjectCytotoxicitypt_PT
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleCyto-genotoxic effects of distinct micro/nanocelluloses in human osteoblastic and mice fibroblastic cellspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleA predictive toxicology approach to characterize potential respiratory effects of functionalized nanocellulose fibres in a co-culture system
oaire.awardTitleInstitute of Nanostructures, Nanomodelling and Nanofabrication
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardTitleChemical Process Engineering and Forest Products Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-PUB%2F32587%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50025%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FCTM-REF%2F30529%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00009%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00009%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00102%2F2020/PT
oaire.citation.conferencePlaceGirona, Espanhapt_PT
oaire.citation.titleIberoamerican Congress on Pulp and Paper Research (CIADICYP), 28 June - 1 July 2022pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream9471 - RIDTI
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameVentura
person.familyNameLouro
person.familyNameSilva
person.givenNameCélia
person.givenNameHenriqueta
person.givenNameMaria Joao
person.identifier2056460
person.identifier157627
person.identifier.ciencia-id6116-89BA-C617
person.identifier.ciencia-id721D-2BB1-7DB1
person.identifier.ciencia-id7710-643D-97A3
person.identifier.orcid0000-0002-0637-2222
person.identifier.orcid0000-0001-9744-7332
person.identifier.orcid0000-0002-6060-0716
person.identifier.scopus-author-id6507971479
person.identifier.scopus-author-id55944437600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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