Repository logo
 
Publication

Distribution and Clinical Significance of HPV16 Variants in Head and Neck Squamous Cell Carcinomas: Data from a Portuguese Cohort and Systematic Review

2023-04-11Journal article Restricted access
Simple item page
dc.contributor.authorCochicho, Daniela
dc.contributor.authorNunes, Alexandra
dc.contributor.authorSobral, Daniel
dc.contributor.authorGomes, João P.
dc.contributor.authorEsteves, Susana
dc.contributor.authorMendonça, Joana
dc.contributor.authorVieira, Luis
dc.contributor.authorMartins, Luís
dc.contributor.authorCunha, Mario
dc.contributor.authorMontalvão, Pedro
dc.contributor.authorMagalhães, Miguel
dc.contributor.authorGil da Costa, Rui M.
dc.contributor.authorFélix, Ana
dc.date.accessioned2024-01-17T16:55:18Z
dc.date.available2024-01-17T16:55:18Z
dc.date.issued2023-04-11
dc.descriptionReviewpt_PT
dc.description.abstractIntroduction: Genomic variants of the human papillomavirus type 16 (HPV16) are thought to play differential roles in the susceptibility to head and neck squamous cell carcinomas (HNSCC) and its biological behaviour. This study aimed to establish the prevalence of HPV16 variants in an HNSCC cohort and associate them with clinical pathological characteristics and patient survival. Methods: We retrieved samples and clinical data from 68 HNSCC patients. DNA samples were available from tumour biopsy at the time of the primary diagnosis. Targeted next-generation sequencing was used to obtain whole-genome sequences, and variants were established based on phylogenetic classification. Results: 74% of samples clustered in lineage A, 5.7% in lineage B, 2.9% in lineage C, and 17.1% in lineage D. Comparative genome analysis revealed 243 single nucleotide variations. Of these, one hundred were previously reported, according to our systematic review. No significant associations with clinical pathological variables or patient survival were observed. The E6 amino acid variations E31G, L83V, and D25E and E7 N29S, associated with cervical cancer, were not observed, except for N29S in a single patient. Conclusion: These results provide a comprehensive genomic map of HPV16 in HSNCC, highlighting tissue-specific characteristics which will help design tailored therapies for cancer patients.pt_PT
dc.description.sponsorshipThis study was financially supported by the Virology Laboratory Pathology Department from the Portuguese Oncology Institute of Lisboa IUIC/1168, with contributions by the GenomePT project (POCI-01-0145-FEDER-022184), supported by COMPETE 2020 - Operational Programme for Competitiveness and Internationalisation (POCI), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Operational Programme (CRESC Algarve2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and by Fundação para a Ciência e a Tecnologia (FCT).pt_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.citationPathobiology. 2023;90(5):333-343. doi: 10.1159/000529723. Epub 2023 Apr 11. Reviewpt_PT
dc.identifier.doi10.1159/000529723pt_PT
dc.identifier.issn1015-2008
dc.identifier.urihttp://hdl.handle.net/10400.18/8918
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherKarger Publisherspt_PT
dc.relationNational Facility for genome sequencing and analysis
dc.relation.publisherversionhttps://karger.com/pat/article-abstract/90/5/333/836884/Distribution-and-Clinical-Significance-of-HPV16?redirectedFrom=fulltextpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectHead and Neck Squamous Cell Carcinomapt_PT
dc.subjectHuman Papillomavirus Type 16pt_PT
dc.subjectHuman Papillomavirus variantspt_PT
dc.subjectOropharynxpt_PT
dc.subjectWhole-genome Sequencingpt_PT
dc.subjectInfecções Sexualmente Transmissíveis
dc.titleDistribution and Clinical Significance of HPV16 Variants in Head and Neck Squamous Cell Carcinomas: Data from a Portuguese Cohort and Systematic Reviewpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNational Facility for genome sequencing and analysis
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9444 - RNIIIE/PINFRA%2F22184%2F2016/PT
oaire.citation.endPage343pt_PT
oaire.citation.issue5pt_PT
oaire.citation.startPage333pt_PT
oaire.citation.titlePathobiologypt_PT
oaire.citation.volume90pt_PT
oaire.fundingStream9444 - RNIIIE
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication3b8a803c-37e5-4354-93f5-6410d7fb2af7
relation.isProjectOfPublication.latestForDiscovery3b8a803c-37e5-4354-93f5-6410d7fb2af7

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
HPV16 Variants HNSCC.pdf
Size:
2.88 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

DDI - Artigos em revistas internacionais
DGH - Artigos em revistas internacionais