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Molecular detection of Rickettsia spp. in ticks and feas collected from rescued hedgehogs (Erinaceus europaeus) in Portugal

2021-03-02Journal article Restricted access
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dc.contributor.authorBarradas, P. F.
dc.contributor.authorMesquita, J. R.
dc.contributor.authorMateus, T. L.
dc.contributor.authorFerreira, P.
dc.contributor.authorAmorim, I.
dc.contributor.authorGartner, F.
dc.contributor.authorSousa, R.
dc.date.accessioned2022-01-31T17:08:31Z
dc.date.available2022-01-31T17:08:31Z
dc.date.issued2021-03-02
dc.description.abstractHedgehogs (e.g., Erinaceus europaeus, E. roumanicus) are wild mammals that frequently are observed near residential areas. The aim of this study was to investigate ticks and feas collected from European hedgehogs in Portugal and to evaluate the prevalence of Rickettsia in those ectoparasites. Ticks and feas were identifed by morphological and molecular methods, and molecular detection by PCR and genotypic characterization of Rickettsia spp. was performed targeting ompB, ompA and gltA gene fragments. In total, 1892 ticks and 213 feas were collected from 33 rescued European hedgehogs captured in seven districts of the north and centre of Portugal. Two tick species were identifed – Rhipicephalus sanguineus accounted for 91% (n=1719) of the total ticks collected and 9% (n=173) were Ixodes hexagonus. All feas were identifed as Archaeopsylla erinacei. Regarding pathogen detection, Rickettsia massiliae DNA was found in 22 of the 212 tested Rh. sanguineus. None of the 48 I. hexagonus tested showed to be positive for rickettsiae. Rickettsia asembonensis DNA was identifed in 55 A. erinacei feas tested (n=117). These results show that European hedgehogs are exposed to R. massiliae transmitted by ticks and to R. asembonensis via feas suggesting that these mammals might be involved in the natural transmission cycle of these Rickettsia species. This study is the frst report of R. asembonensis in feas in Portugal.pt_PT
dc.description.sponsorshipP. Barradas (SFRH/BD/116449/2016) acknowledges the Portuguese Foundation for Science and Technology (FCT) for fnancial support. IPATIMUP integrates the i3S Research Unit, which is partially supported by FEDER funds through the Operational Programme for Competitiveness FactorsCOMPETE and National Funds through the FCT, under the project number PEst-C/SAU/LA0003/2013.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationExp Appl Acarol. 2021 Mar;83(3):449-460. doi: 10.1007/s10493-021-00600-y. Epub 2021 Mar 2pt_PT
dc.identifier.doi10.1007/s10493-021-00600-ypt_PT
dc.identifier.issn0168-8162
dc.identifier.urihttp://hdl.handle.net/10400.18/7898
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationThe role of dogs and their susceptibility to the infection by Spotted Fever Rickettsiae. Characterization of the immunopathology of Rickettsia conorii infection
dc.relation.publisherversionhttps://link.springer.com/article/10.1007%2Fs10493-021-00600-ypt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectRickettsia asembonensispt_PT
dc.subjectArchaeopsylla erinaceipt_PT
dc.subjectRhipicephalus sanguineuspt_PT
dc.subjectHedgehogpt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.subjectPortugalpt_PT
dc.titleMolecular detection of Rickettsia spp. in ticks and feas collected from rescued hedgehogs (Erinaceus europaeus) in Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleThe role of dogs and their susceptibility to the infection by Spotted Fever Rickettsiae. Characterization of the immunopathology of Rickettsia conorii infection
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F116449%2F2016/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/COMPETE/PEst-C%2FSAU%2FLA0003%2F2013/PT
oaire.citation.endPage460pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage449pt_PT
oaire.citation.titleExperimental and Applied Acarologypt_PT
oaire.citation.volume83pt_PT
oaire.fundingStreamCOMPETE
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication0aa5f01d-c6d6-4dc4-9ad4-308f6640207e
relation.isProjectOfPublicationf37b50c3-33c1-479a-a758-f45aa5b3d99f
relation.isProjectOfPublication.latestForDiscovery0aa5f01d-c6d6-4dc4-9ad4-308f6640207e

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