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Combination anthelmintic/antioxidant activity against Schistosoma Mansoni

2019-02-05Journal article Open access
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dc.contributor.authorGouveia, Maria João
dc.contributor.authorBrindley, Paul J.
dc.contributor.authorRinaldi, Gabriel
dc.contributor.authorGärtner, Fátima
dc.contributor.authorda Costa, José Manuel Correia
dc.contributor.authorVale, Nuno
dc.date.accessioned2020-05-09T15:06:35Z
dc.date.available2020-05-09T15:06:35Z
dc.date.issued2019-02-05
dc.description.abstractSchistosomiasis is a major neglected tropical disease. Treatment for schistosomiasis with praziquantel (PZQ), which is effective against the parasite, by itself is not capable to counteract infection-associated disease lesions including hepatic fibrosis. There is a pressing need for novel therapies. Due to their biological properties, antioxidant biomolecules might be useful in treating and reverting associated pathological sequelae. Here, we investigated a novel therapy approach based on a combination of anthelmintic drugs with antioxidant biomolecules. We used a host-parasite model involving Bioamphalaria glabrata and newly transformed schistosomula (NTS) of Schistosoma mansoni. For in vitro drug screening assays, was selected several antioxidants and evaluated not only antischistosomal activity but also ability to enhance activity of the anthelmintic drugs praziquantel (PZQ) and artesunate (AS). The morphological alterations induced by compounds alone/combined were assessed on daily basis using an inverted and automated microscope to quantify NTS viability by a fluorometric-based method. The findings indicated that not only do some antioxidants improve antischistosomal activity of the two anthelmintics, but they exhibit activity per se, leading to high mortality of NTS post-exposure. The combination index (CI) of PZQ + Mel (CI = 0.80), PZQ + Resv (CI = 0.74), AS + Resv (CI = 0.34), AS + NAC (CI = 0.89), VDT + Flav (CI = 1.03) and VDT + Resv (CI = 1.06) reveal that they display moderate to strong synergism. The combination of compounds with discrete mechanisms of action might provide a valuable adjunct to contribution for treatment of schistosomiasis-associated disease.pt_PT
dc.description.sponsorshipThis work was financed by FEDER—Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020—Operational Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, and by Portuguese funds through FCT—Fundação para a Ciência e a Tecnologia, in the framework of the projects “Institute for Research and Innovation in Health Sciences” (POCI-01-0145-FEDER-007274). NV also acknowledges support from FCT and FEDER (European Union), award number IF/00092/2014/CP1255/CT0004.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBiomolecules. 2019 Feb 5;9(2):54. doi: 10.3390/biom9020054.pt_PT
dc.identifier.doi10.3390/biom9020054pt_PT
dc.identifier.issn2218-273X
dc.identifier.urihttp://hdl.handle.net/10400.18/6633
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2218-273X/9/2/54pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnimalspt_PT
dc.subjectAntineoplastic Agentspt_PT
dc.subjectAntioxidantspt_PT
dc.subjectAntiprotozoal Agentspt_PT
dc.subjectCell Survivalpt_PT
dc.subjectSchistosoma mansonipt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleCombination anthelmintic/antioxidant activity against Schistosoma Mansonipt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue2pt_PT
oaire.citation.startPage54pt_PT
oaire.citation.titleBiomoleculespt_PT
oaire.citation.volume9pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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