Publication
Identification of polysaccharide capsules among extensively drug-resistant genitourinary Haemophilus parainfluenzae isolates
| dc.contributor.author | González-Díaz, Aida | |
| dc.contributor.author | Tubau, Fe | |
| dc.contributor.author | Pinto, Miguel | |
| dc.contributor.author | Sierra, Yanik | |
| dc.contributor.author | Cubero, Meritxell | |
| dc.contributor.author | Càmara, Jordi | |
| dc.contributor.author | Ayats, Josefina | |
| dc.contributor.author | Bajanca-Lavado, Paula | |
| dc.contributor.author | Ardanuy, Carmen | |
| dc.contributor.author | Marti, Sara | |
| dc.date.accessioned | 2020-04-25T11:17:01Z | |
| dc.date.available | 2020-04-25T11:17:01Z | |
| dc.date.issued | 2019-03-14 | |
| dc.description | Free PMC article: https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/30872664/ | pt_PT |
| dc.description.abstract | The human commensal Haemophilus parainfluenzae is emerging as an opportunistic multidrug-resistant pathogen. The objectives of this work were to characterise a new capsular operon of extensively drug-resistant (XDR) H. parainfluenzae clinical isolates and study their resistance mechanisms using whole-genome sequencing. All strains were resistant to: ß-lactams, via amino acid changes in PBP3 (S385T, I442F, V511A, N526K and V562I); quinolones, by alterations in GyrA (S84F and D88Y) and ParC (S84F and S138T); chloramphenicol, through the presence of catS; macrolides, via the presence of mel and mef(E)-carrying MEGA element; and tetracycline, through the presence of tet(M) and/or tet(B). Phylogenetic analysis revealed high genomic diversity when compared to the H. parainfluenzae genomes available on the NCBI, the isolates from this study being closely related to the Swiss XDR AE-2096513. A full capsular operon showing homology to that of H. influenzae was identified, in accordance with the observation of a capsular structure by TEM. This study describes for the first time a capsular operon in H. parainfluenzae, a major determinant of pathogenicity that may contribute to increased virulence in XDR clinical isolates. Moreover, phylogenetic analysis suggests the possible spread of an XDR-encapsulated strain in Europe. | pt_PT |
| dc.description.sponsorship | This study has been funded by Instituto de Salud Carlos III through the Projects from the Fondo de Investigaciones Sanitarias “PI16/00977” and CIBER de Enfermedades Respiratorias (CIBERES - CB06/06/0037), and Co-funded by the European Regional Development Fund/European Social Fund (ERDF/ESF, “Investing in your future”). | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Sci Rep . 2019 Mar 14;9(1):4481. doi: 10.1038/s41598-019-40812-2 | pt_PT |
| dc.identifier.doi | 10.1038/s41598-019-40812-2 | pt_PT |
| dc.identifier.issn | 2045-2322 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/6519 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Nature Research | pt_PT |
| dc.relation.publisherversion | https://www.nature.com/articles/s41598-019-40812-2 | pt_PT |
| dc.subject | Haemophilus parainfluenzae | pt_PT |
| dc.subject | Extensively Drug-resistat | pt_PT |
| dc.subject | Genitourinary Tract | pt_PT |
| dc.subject | Capsule | pt_PT |
| dc.subject | Infecções Respiratórias | pt_PT |
| dc.title | Identification of polysaccharide capsules among extensively drug-resistant genitourinary Haemophilus parainfluenzae isolates | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.issue | 1 | pt_PT |
| oaire.citation.startPage | 4481 | pt_PT |
| oaire.citation.title | Scientific Reports | pt_PT |
| oaire.citation.volume | 9 | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |