Publicação
Uropathogenic Escherichia coli in a Diabetic Dog with Recurrent UTIs: Genomic Insights and the Impact of Glucose and Antibiotics on Biofilm Formation
| dc.contributor.author | Rodrigues, Inês C. | |
| dc.contributor.author | Ribeiro-Almeida, Marisa | |
| dc.contributor.author | Campos, Joana | |
| dc.contributor.author | Silveira, Leonor | |
| dc.contributor.author | Leite-Martins, Liliana | |
| dc.contributor.author | Ribeiro, Jorge | |
| dc.contributor.author | da Costa, Paula Martins | |
| dc.contributor.author | Prata, Joana C. | |
| dc.contributor.author | Pista, Ângela | |
| dc.contributor.author | Costa, Paulo Martins da | |
| dc.date.accessioned | 2026-01-21T16:23:09Z | |
| dc.date.available | 2026-01-21T16:23:09Z | |
| dc.date.issued | 2025-08-20 | |
| dc.description | This article belongs to the Special Issue Antimicrobial Resistance and the Use of Antibiotics in Animals. | |
| dc.description.abstract | Recurrent urinary tract infections (UTIs) pose a significant clinical challenge in both human and veterinary medicine, due to antibiotic-resistant and biofilm-forming bacteria. We hypothesized that high glucose levels in diabetic animals enhance biofilm formation and reduce antibiotic efficacy, promoting infection persistence. This study analyzed Escherichia coli from a diabetic female Labrador Retriever with recurrent UTIs over 18 months, focusing on antimicrobial resistance, biofilm-forming capacity, and genomic characterization. Most isolates (9/11) were resistant to ampicillin and fluoroquinolones. Whole genome sequencing of six selected isolates revealed that they belonged to the multidrug-resistant ST1193 lineage, a globally emerging clone associated with persistent infections. Phylogenetic analysis revealed clonal continuity across six UTI episodes, with two distinct clones identified: one during a coinfection in the second episode and another in the last episode. High-glucose conditions significantly enhanced biofilm production and dramatically reduced antibiotic susceptibility, as evidenced by a marked increase in minimum biofilm inhibitory concentrations (MBICs), which were at least 256-fold higher than the corresponding minimum inhibitory concentration (MIC). Sulfamethoxazole-trimethoprim demonstrated the strongest antibiofilm activity, though this was attenuated in glucose-supplemented environments. This research highlights the clinical relevance of glucosuria in diabetic patients and emphasizes the need for therapeutic strategies targeting biofilm-mediated antibiotic tolerance to improve the management of recurrent UTIs. | eng |
| dc.identifier.citation | Microorganisms. 2025 Aug 20;13(8):1946. doi: 10.3390/microorganisms13081946 | |
| dc.identifier.doi | 10.3390/microorganisms13081946 | |
| dc.identifier.eissn | 2076-2607 | |
| dc.identifier.pmid | 40871450 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/10734 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | MDPI | |
| dc.relation.hasversion | https://www.mdpi.com/2076-2607/13/8/1946 | |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject | UTI | |
| dc.subject | Uropathogenic Escherichia Coli | |
| dc.subject | Antibiotic Pressure | |
| dc.subject | Biofilm | |
| dc.subject | Resistência aos Antimicrobianos | |
| dc.title | Uropathogenic Escherichia coli in a Diabetic Dog with Recurrent UTIs: Genomic Insights and the Impact of Glucose and Antibiotics on Biofilm Formation | eng |
| dc.type | journal article | |
| dcterms.references | https://www.mdpi.com/article/10.3390/microorganisms13081946/s1 | |
| dspace.entity.type | Publication | |
| oaire.citation.issue | 8 | |
| oaire.citation.startPage | 1946 | |
| oaire.citation.title | Microorganisms | |
| oaire.citation.volume | 13 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 |