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Thymus mastichina (L.) L. and Cistus ladanifer L. for skin application: chemical characterization and in vitro bioactivity assessment

2022-10-13Journal article Restricted access
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dc.contributor.authorOliveira, Ana S.
dc.contributor.authorRolo, Joana
dc.contributor.authorGaspar, Carlos
dc.contributor.authorRamos, Leonor
dc.contributor.authorCavaleiro, Carlos
dc.contributor.authorSalgueiro, Lígia
dc.contributor.authorPalmeira-de-Oliveira, Rita
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorMartinez-de-Oliveira, José
dc.contributor.authorPalmeira-de-Oliveira, Ana
dc.date.accessioned2023-03-22T16:20:51Z
dc.date.available2023-03-22T16:20:51Z
dc.date.issued2022-10-13
dc.description.abstractEthnopharmacological relevance: Thymus mastichina (L.) L. (TM) and Cistus ladanifer L. (CL) are two Portuguese autochthonous species with traditional skin application in folk medicine. TM is majorly known for its antiseptic and wound healing properties, as an external anti-inflammatory agent and for its application in folk cosmetics and hygiene products. Its use in acne vulgaris has also been reported. CL is traditionally used in remedies for wounds, ulcers and other skin ailments such as psoriasis and eczema. Its application has been found useful due to its anti-inflammatory, astringent, wound healing and antiseptic properties. Aim of the study: With this work, we aimed to investigate relevant bioactivities related with the traditional application of TM and CL essential oils (EOs) and hydrolates (by-products of EO production) in skin ailments. Specifically their in vitro antioxidant, anti-inflammatory, cytotoxic, wound healing and antimicrobial properties were evaluated. The chemical composition of both EOs and respective hydrolates was also characterized. Materials and methods: Chemical characterization of EOs and hydrolates was performed by GC-FID and GC-MS. Cellular biocompatibility was evaluated using the MTT assay in macrophages (RAW 264.7) and fibroblasts (L929) cell lines. Anti-inflammatory activity was investigated by studying nitric oxide (NO) production by macrophages with Griess reagent. Wound healing potential was evaluated with the scratch-wound assay. The antioxidant potential was studied by the DPPH scavenging method. Antimicrobial activity was evaluated by broth microdilution assay against relevant microbial strains and skin pathogens, namely Staphylococcus aureus, Staphylococcus epidermidis, Cutibacterium acnes, Pseudomonas aeruginosa, Escherichia coli, Candida albicans and Aspergillus brasiliensis. Results: The major compounds present in TM and CL EOs were 1,8-cineole and α-pinene, respectively. 1,8-cineole and E-pinocarveol were the major compounds in the correspondent hydrolates. CL EO presented the highest anti-inflammatory potential [EC50 = 0.002% (v/v)], still with significant cytotoxicity [IC50 = 0.012% (v/v)]. TM preparations presented anti-inflammatory potential, also presenting higher biocompatibility. The same profile was present on fibroblasts regarding biocompatibility of the tested preparations. CL EO and hydrolate increased fibroblasts' migration by 155.7% and 148.4%, respectively. TM hydrolate presented a milder activity than CL hydrolate, but wound healing potential was still present, increasing cell migration by 125.1%. All preparations presented poor antioxidant capacity. CL EO presented higher antimicrobial activity, with MICs ranging from 0.06% (v/v) to 2% (v/v), against different microorganisms. Conclusions: Anti-inflammatory and skin repairing potential were present for CL preparations. TM hydrolate presented an interesting biocompatible profile on both cell lines, also presenting anti-inflammatory potential. Furthermore, EOs from both species presented antimicrobial activity against a panel of different microorganisms. These in vitro bioactivities support some of their traditional skin applications, specifically regarding their antiseptic, wound healing and anti-inflammatory uses.pt_PT
dc.description.sponsorshipThis work was supported by ‘INOVEP project – Innovation with Plant Extracts’ [project number 33815], I&DT projects for companies in collaboration with scientific entities funded by Centro2020; and by project Coop4PAM, funded by PO Interreg V-A Espanha-Portugal (POCTEP) 2014–2020. It was also part of Forest for the Future - F4F project funded by [CENTRO-08-5864-FSE-000031]. The work was developed within the scope of the CICS-UBI projects [UIDB/00709/ 2020] and [UIDP/00709/2020], financed by national funds through the Portuguese Foundation for Science and Technology/MCTES (FCT). Financial support was also provided by FCT through a Ph.D. fellowship [SFRH//BD/136192/2018] awarded to ASO and through a fellowship awarded to JR [SFRH/BPD/115145/2016].pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Ethnopharmacol. 2023 Feb 10;302(Pt A):115830. doi: 10.1016/j.jep.2022.115830. Epub 2022 Oct 13.pt_PT
dc.identifier.doi10.1016/j.jep.2022.115830pt_PT
dc.identifier.issn0378-8741
dc.identifier.urihttp://hdl.handle.net/10400.18/8599
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationHealth Sciences Research Centre
dc.relationHealth Sciences Research Centre
dc.relationalterado para: “Portuguese Natural Resources as Modulators of Acne Vulgaris Hallmarks” Nonspecific vulvovaginitis: the putative ethiopatologic role of House Dust Mites
dc.relationRole of the native vaginal flora in recurrent vulvovaginal candidosis
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0378874122008698?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAntimicrobial Activitypt_PT
dc.subjectCistus ladanifer L.pt_PT
dc.subjectInflammationpt_PT
dc.subjectThymus mastichina (L.) Lpt_PT
dc.subjectWound Healingpt_PT
dc.subjectToxicologiapt_PT
dc.titleThymus mastichina (L.) L. and Cistus ladanifer L. for skin application: chemical characterization and in vitro bioactivity assessmentpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitlealterado para: “Portuguese Natural Resources as Modulators of Acne Vulgaris Hallmarks” Nonspecific vulvovaginitis: the putative ethiopatologic role of House Dust Mites
oaire.awardTitleRole of the native vaginal flora in recurrent vulvovaginal candidosis
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F136192%2F2018/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F115145%2F2016/PT
oaire.citation.issuePt Apt_PT
oaire.citation.startPage115830pt_PT
oaire.citation.titleJournal of Ethnopharmacologypt_PT
oaire.citation.volume302pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationced21566-cfde-4640-afff-0536a07c1f8f
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