Publication
Haemophilus influenzae Carriage among Healthy Children in Portugal, 2015-2019
| dc.contributor.author | Bajanca-Lavado, Maria Paula | |
| dc.contributor.author | Cavaco, Luís | |
| dc.contributor.author | Fernandes, Mariana | |
| dc.contributor.author | Touret, Tiago | |
| dc.contributor.author | Candeias, Catarina | |
| dc.contributor.author | Simões, Alexandra S. | |
| dc.contributor.author | Sá-Leão, Raquel | |
| dc.date.accessioned | 2022-12-02T15:47:32Z | |
| dc.date.available | 2022-12-02T15:47:32Z | |
| dc.date.issued | 2022-10-04 | |
| dc.description | This article belongs to the Special Issue Haemophilus influenzae: New Insights in Epidemiology of Disease. | pt_PT |
| dc.description.abstract | Haemophilus influenzae is an important cause of mucosal and invasive infections and a common colonizer of the upper respiratory tract. As there are no recent data on H. influenzae carriage in Portugal, we aimed to characterize carriage samples and investigate possible parallelisms with disease isolates. Between 2016–2019, 1524 nasopharyngeal samples were obtained from children (0–6 years) attending day-care. H. influenzae were serotyped and screened for β-lactamase production. Strains producing β-lactamase and/or those that were encapsulated were further characterized by antibiotype; encapsulated strains were also investigated for MLST and the presence of antimicrobial resistance and virulence genes (extracted from whole genome sequencing). The overall carriage rate was 84.1%. Most isolates (96.7%) were nonencapsulated. Encapsulated strains were of serotypes f (1.8%), e (1.1%), a (0.3%), and b (0.1%). MLST showed clonality within serotypes. Although the lineages were the same as those that were described among disease isolates, colonization isolates had fewer virulence determinants. Overall, 7.5% of the isolates were β-lactamase positive; one isolate had blaTEM-82, which has not been previously described in H. influenzae. A single isolate, which was identified as H. parainfluenzae, had an incomplete f-like cap locus. In conclusion, circulation of serotype b is residual. The few encapsulated strains are genetically related to disease-causing isolates. Thus, surveillance of H. influenzae carriage should be maintained. | pt_PT |
| dc.description.sponsorship | This work was partially supported by projects LISBOA-01-0145-FEDER (Microbiologia Molecular, Estrutural e Celular, funded by FEDER through COMPETE2020—Programa Operacional Competitividade e Internacionalização), LISBOA-01-0145-FEDER-016417 (ONEIDA co-funded by Fundos Europeus Estruturais e de Investimento, Programa Operacional Regional Lisboa 2020 and Fundação para a Ciência e a Tecnologia (FCT)), WI230921 from Pfizer Portugal (to RSL), and National Institute of Health, Lisbon, Portugal. CC was supported by grant from Fundação para a Ciência e a Tecnologia, Portugal. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Microorganisms. 2022 Oct 4;10(10):1964. doi: 10.3390/microorganisms10101964 | pt_PT |
| dc.identifier.doi | 10.3390/microorganisms10101964 | pt_PT |
| dc.identifier.issn | 2076-2607 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/8375 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | MDPI | pt_PT |
| dc.relation.publisherversion | https://www.mdpi.com/2076-2607/10/10/1964 | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Haemophilus influenzae | pt_PT |
| dc.subject | Serotype | pt_PT |
| dc.subject | MLST | pt_PT |
| dc.subject | Carriage | pt_PT |
| dc.subject | Surveillance | pt_PT |
| dc.subject | Antimicrobial Resistance | pt_PT |
| dc.subject | Capsule | pt_PT |
| dc.subject | Whole Genome Sequencing | pt_PT |
| dc.subject | Day-care Center | pt_PT |
| dc.subject | Portugal | pt_PT |
| dc.subject | Infecções Respiratórias | pt_PT |
| dc.title | Haemophilus influenzae Carriage among Healthy Children in Portugal, 2015-2019 | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.issue | 10 | pt_PT |
| oaire.citation.startPage | 1964 | pt_PT |
| oaire.citation.title | Microorganisms | pt_PT |
| oaire.citation.volume | 10 | pt_PT |
| rcaap.embargofct | Acesso de acordo com política editorial da revista. | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |