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Identifying phage Lysins through genomic analysis of prophages from Acinetobacter baumannii

2025-04-01Artigo científico Acesso aberto
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dc.contributor.authorRaposo, Maria Leonor
dc.contributor.authorPimentel, Ana Carolina
dc.contributor.authorManageiro, Vera
dc.contributor.authorDuarte, Aida
dc.contributor.authorCaniça, Manuela
dc.contributor.authorVale, Filipa F.
dc.date.accessioned2026-02-06T15:09:39Z
dc.date.available2026-02-06T15:09:39Z
dc.date.issued2025-04-01
dc.description.abstractAcinetobacter baumannii is a Gram-negative opportunistic pathogen, responsible for nosocomial infections worldwide. In recent years, this microorganism has acquired resistance to various antibiotics, prompting the World Health Organization (WHO) to declare carbapenem-resistant A. baumannii (CRAB) a critical priority microorganism requiring urgent attention and the development of new therapeutic options. Here, we screened for prophages in 158 genomes of A. baumannii, comprising 139 complete genomes from the Bacterial and Viral Bioinformatics Resource Center (BV-BRC), and 19 newly sequenced clinical isolates. Additionally, we conducted phylogenetic analyses of prophages, highlighting their diversity and local clustering. The analyzed genomes harbored at least two prophage regions, resulting in the identification of a total of 950 prophage regions, of which 348 were considered complete prophages through software analysis and manual curation, while the remainder may represent prophage remnants. The complete prophages ranged from 28.6 to 103.9 kbp, with an average GC content of 39%. Based on genomic similarity, only 18 complete prophages were taxonomically classified to the genus Vieuvirus. Among all identified complete prophages, we identified 166 genes encoding for putative lysins, while prophage regions that were not considered complete could also harbor putative lysins. These findings highlight the abundance of prophage-encoded lysins in A. baumannii genomes, which are promising therapeutic agents for combating A. baumannii infections, particularly in the face of rising antibiotic resistance.eng
dc.description.sponsorshipThe author(s) declare that financial support was received for the research and/or publication of this article. This work was partially supported by National funds from FCT, projects UIDB/04138/2020 and UIDP/04138/2020. F.F.V. hold project grans (projects PTDC/BTM-SAL/28978/2017) from FCT that supported this work. Work supported by UIDB/04046/2020 (DOI: 10.54499/UIDB/04046/2020) and UIDP/04046/2020 (DOI: 10.54499/UIDP/04046/2020) Centre grants from FCT, Portugal (to BioISI).
dc.identifier.citationFront Microbiol. 2025 Apr 1:16:1532950. doi: 10.3389/fmicb.2025.1532950. eCollection 2025
dc.identifier.doi10.3389/fmicb.2025.1532950
dc.identifier.eissn1664-302X
dc.identifier.pmid40236489
dc.identifier.urihttp://hdl.handle.net/10400.18/10835
dc.language.isoeng
dc.peerreviewedyes
dc.publisherFrontiers Media
dc.relationResearch Institute for Medicines
dc.relationResearch Institute for Medicines
dc.relationBiosystems and Integrative Sciences Institute
dc.relationBiosystems and Integrative Sciences Institute
dc.relation.hasversionhttps://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2025.1532950/full
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAcinetobacter baumannii
dc.subjectAntibiotic Resistance
dc.subjectLysins
dc.subjectPhylogeny
dc.subjectProphages
dc.subjectResistência aos Antimicrobianos
dc.titleIdentifying phage Lysins through genomic analysis of prophages from Acinetobacter baumanniieng
dc.typejournal article
dcterms.referenceshttps://www.frontiersin.org/api/v4/articles/1532950/file/Table_1.xlsx/1532950_table_1/1
dspace.entity.typePublication
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleBiosystems and Integrative Sciences Institute
oaire.awardTitleBiosystems and Integrative Sciences Institute
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04138%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04138%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04046%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04046%2F2020/PT
oaire.citation.startPage1532950
oaire.citation.titleFrontiers in Microbiology
oaire.citation.volume16
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
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