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The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

2019-04-20Journal article Restricted access
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dc.contributor.authorGajski, Goran
dc.contributor.authorŽegura, Bojana
dc.contributor.authorLadeira, Carina
dc.contributor.authorNovak, Matjaž
dc.contributor.authorSramkova, Monika
dc.contributor.authorPourrut, Bertrand
dc.contributor.authorDel Bo’, Cristian
dc.contributor.authorMilić, Mirta
dc.contributor.authorGutzkow, Kristine Bjerve
dc.contributor.authorCosta, Solange
dc.contributor.authorDusinska, Maria
dc.contributor.authorBrunborg, Gunnar
dc.contributor.authorCollins, Andrew
dc.date.accessioned2020-05-06T16:17:40Z
dc.date.available2020-05-06T16:17:40Z
dc.date.issued2019-04-20
dc.description.abstractThe comet assay has become one of the methods of choice for the evaluation and measurement of DNA damage. It is sensitive, quick to perform and relatively affordable for the evaluation of DNA damage and repair at the level of individual cells. The comet assay can be applied to virtually any cell type derived from different organs and tissues. Even though the comet assay is predominantly used on human cells, the application of the assay for the evaluation of DNA damage in yeast, plant and animal cells is also quite high, especially in terms of biomonitoring. The present extensive overview on the usage of the comet assay in animal models will cover both terrestrial and water environments. The first part of the review was focused on studies describing the comet assay applied in invertebrates. The second part of the review, (Part 2) will discuss the application of the comet assay in vertebrates covering cyclostomata, fishes, amphibians, reptiles, birds and mammals, in addition to chordates that are regarded as a transitional form towards vertebrates. Besides numerous vertebrate species, the assay is also performed on a range of cells, which includes blood, liver, kidney, brain, gill, bone marrow and sperm cells. These cells are readily used for the evaluation of a wide spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of vertebrate models and their role in environmental biomonitoring will also be discussed as well as the comparison of the use of the comet assay in vertebrate and human models in line with ethical principles. Although the comet assay in vertebrates is most commonly used in laboratory animals such as mice, rats and lately zebrafish, this paper will only briefly review its use regarding laboratory animal models and rather give special emphasis to the increasing usage of the assay in domestic and wildlife animals as well as in various ecotoxicological studies.pt_PT
dc.description.sponsorshipThis work was supported by the European Cooperation in Science and Technology (CA COST Action CA15132 – The comet assay as a human biomonitoring tool (hCOMET)).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMutat Res. Jul-Sep 2019;781:130-164. doi: 10.1016/j.mrrev.2019.04.002. Epub 2019 Apr 20. Reviewpt_PT
dc.identifier.doi10.1016/j.mrrev.2019.04.002pt_PT
dc.identifier.issn1383-5742
dc.identifier.urihttp://hdl.handle.net/10400.18/6605
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S138357421830108X?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectAnimalspt_PT
dc.subjectComet Assaypt_PT
dc.subjectDNA Damagept_PT
dc.subjectEnvironmental Monitoringpt_PT
dc.subjectHumanspt_PT
dc.subjectModels, Animalpt_PT
dc.subjectVertebratespt_PT
dc.subjectWhalespt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleThe comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage164pt_PT
oaire.citation.startPage130pt_PT
oaire.citation.titleMutation Research - Reviewspt_PT
oaire.citation.volume781pt_PT
rcaap.embargofctDe acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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