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Unveiling Antibiotic Resistance, Clonal Diversity, and Biofilm Formation in Isolated from Healthy Swine in Portugal

2024-04-09Journal article Open access
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dc.contributor.authorSilva, Adriana
dc.contributor.authorSilva, Vanessa
dc.contributor.authorDapkevicius, Maria de Lurdes Enes
dc.contributor.authorAzevedo, Mónica
dc.contributor.authorCordeiro, Rui
dc.contributor.authorPereira, José Eduardo
dc.contributor.authorValentão, Patrícia
dc.contributor.authorFalco, Virgílio
dc.contributor.authorIgrejas, Gilberto
dc.contributor.authorCaniça, Manuela
dc.contributor.authorPoeta, Patrícia
dc.date.accessioned2025-02-17T16:46:00Z
dc.date.available2025-02-17T16:46:00Z
dc.date.issued2024-04-09
dc.description.abstractEscherichia coli, a commensal microorganism found in the gastrointestinal tract of human and animal hosts, plays a central role in agriculture and public health. Global demand for animal products has promoted increased pig farming, leading to growing concerns about the prevalence of antibiotic-resistant E. coli strains in swine populations. It should be noted that a significant portion of antibiotics deployed in swine management belong to the critically important antibiotics (CIA) class, which should be reserved for human therapeutic applications. This study aimed to characterize the prevalence of antibiotic resistance, genetic diversity, virulence characteristics, and biofilm formation of E. coli strains in healthy pigs from various farms across central Portugal. Our study revealed high levels of antibiotic resistance, with resistance to tetracycline, ampicillin, tobramycin, and trimethoprim-sulfamethoxazole. Multidrug resistance is widespread, with some strains resistant to seven different antibiotics. The ampC gene, responsible for broad-spectrum resistance to cephalosporins and ampicillin, was widespread, as were genes associated with resistance to sulfonamide and beta-lactam antibiotics. The presence of high-risk clones, such as ST10, ST101, and ST48, are a concern due to their increased virulence and multidrug resistance profiles. Regarding biofilm formation, it was observed that biofilm-forming capacity varied significantly across different compartments within pig farming environments. In conclusion, our study highlights the urgent need for surveillance and implementation of antibiotic management measures in the swine sector. These measures are essential to protect public health, ensure animal welfare, and support the swine industry in the face of the growing global demand for animal products.eng
dc.description.sponsorshipThis work was supported by the projects UIDB/CVT/00772/2020 and LA/P/0059/2020 funded by the Portuguese Foundation for Science and Technology (FCT). This work received support and help from FCT/MCTES (LA/P/0008/2020 DOI 10.54499/LA/P/0008/2020, UIDP/50006/2020 DOI 10.54499/UIDP/50006/2020 and UIDB/50006/2020 DOI 10.54499/UIDB/50006/2020). Adriana Silva is grateful to FCT (Fundação para a Ciência e a Tecnologia) for financial support through the PhD grant SFRH/ BD/04576/2020.
dc.identifier.citationPathogens. 2024 Apr 9;13(4):305. doi: 10.3390/pathogens13040305
dc.identifier.doi10.3390/pathogens13040305
dc.identifier.eissn2076-0817
dc.identifier.pmid38668260
dc.identifier.urihttp://hdl.handle.net/10400.18/10356
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationAssociate Laboratory for Animal and Veterinary Sciences
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relation.hasversionhttps://www.mdpi.com/2076-0817/13/4/305
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectEscherichia coli
dc.subjectAnimal Production
dc.subjectAntimicrobial Resistance
dc.subjectFood Safety
dc.subjectMulti-resistance
dc.subjectSwine Farms
dc.subjectResistência aos Antimicrobianos
dc.titleUnveiling Antibiotic Resistance, Clonal Diversity, and Biofilm Formation in Isolated from Healthy Swine in Portugaleng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAssociate Laboratory for Animal and Veterinary Sciences
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0059%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0008%2F2020/PT
oaire.citation.issue4
oaire.citation.startPage305
oaire.citation.titlePathogens
oaire.citation.volume13
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isProjectOfPublication93de5af7-dedc-4696-ae11-592584b020a8
relation.isProjectOfPublication72e49150-e3b3-4b5b-8e37-a5e3c312a767
relation.isProjectOfPublication.latestForDiscovery93de5af7-dedc-4696-ae11-592584b020a8

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