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Protein content and amino acid profile in Portuguese rice

dc.contributor.authorMota, Carla
dc.contributor.authorCabral, Margarida
dc.contributor.authorMauro, Raul
dc.contributor.authorTorres, Duarte
dc.contributor.authorSantos, Mariana
dc.contributor.authorAndré, Catarina
dc.contributor.authorCastanheira, Isabel
dc.date.accessioned2014-01-31T14:43:08Z
dc.date.available2014-01-31T14:43:08Z
dc.date.issued2013-09-22
dc.description.abstractAccording to INE, between 2010 and 2011, rice (Oryza sativa) availability in Portugal was 15.8 kg per capita per day, which represents the highest in Europe [1]. Rice amino acids profile evaluation is important to define the nutritional quality of different species contributing to choice of the varieties of rice to grow. This study aimed to quantify the total protein and amino acids profile in 15 cultivated accessions selected from the two major subspecies, Japonica and Indica, grown in Portuguese Sado and Ribatejo regions between 2009 and 2011. Total protein was determined by Kjeldahl method and the content of AA was measured by an Ultra Performance Liquid Chromatography of ACQUITY ™ UPLC ® (Waters) system equipped with a photodiode array detector (DAD) with a pre-derivatisation column 6-N-aminoquinolil hidroxisuccinimidil carbamate, after microwave acid hydrolysis. Analysis of the results emphasizes that there was no significant difference between Japonica varieties (6.4 ± 0.5 g/100g) and Indica (7.0 ± 1.6 g/100g) in relation to total protein content. Amino acids score, as defined by WHO/FAO/UNU [2], was between 0.55 (lysine) and 3.24 (aromatic amino acids). Lysine was the only limiting AA (score <1). No significant differences were observed in amino content between subspecies, cultivation region and year of crop. The obtained results may be aggregated and include in the Portuguese Food Composition Databank and EuroFIR Food Platform [3]. Research on wholegrain and husk rice properties is underway and will be discussed.por
dc.description.sponsorshipAgência Portuguesa de Inovaçãopor
dc.identifier.urihttp://hdl.handle.net/10400.18/1900
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherInstituto Nacional de Saúde Doutor Ricardo Jorge, IPpor
dc.relationValCAP - 23290por
dc.subjectAminoAcid Profilepor
dc.subjectFood Compositionpor
dc.subjectRicepor
dc.subjectProteinpor
dc.subjectComposição dos Alimentospor
dc.titleProtein content and amino acid profile in Portuguese ricepor
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceLisboa, Portugalpor
oaire.citation.title3rd IMEKO TC8, TC23, TC24 - Symposium on Traceability in Chemical, Food and Nutrition Measurements, 22 setembro 2013por
rcaap.rightsrestrictedAccesspor
rcaap.typeconferenceObjectpor

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