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Adaptation of Brucella melitensis Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Method

2022-07-20Journal article Open access
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dc.contributor.authorTscherne, A.
dc.contributor.authorMantel, E.
dc.contributor.authorBoskani, T.
dc.contributor.authorBudniak, S.
dc.contributor.authorElschner, M.
dc.contributor.authorFasanella, A.
dc.contributor.authorFeruglio, S.L.
dc.contributor.authorGalante, D.
dc.contributor.authorGiske, C.G.
dc.contributor.authorGrunow, Roland
dc.contributor.authorHenczko, Judit
dc.contributor.authorHinz, Christin
dc.contributor.authorIwaniak, Wojciech
dc.contributor.authorJacob, Daniela
dc.contributor.authorKedrak-Jablonska, Agnieszka
dc.contributor.authorJensen, Veronica K.
dc.contributor.authorJohansen, Tone B.
dc.contributor.authorKahlmeter, Gunnar
dc.contributor.authorManzulli, Viviana
dc.contributor.authorMatuschek, Erika
dc.contributor.authorMelzer, Falk
dc.contributor.authorNuncio, Maria S.
dc.contributor.authorPapaparaskevas, Joseph
dc.contributor.authorPelerito, Ana
dc.contributor.authorSolheim, Margrete
dc.contributor.authorThomann, Susanne
dc.contributor.authorTsakris, Athanasios
dc.contributor.authorWahab, Tara
dc.contributor.authorWeiner, Marcin
dc.contributor.authorZoeller, Lothar
dc.contributor.authorZange, Sabine
dc.contributor.authorEMERGE AST Working Group
dc.date.accessioned2022-12-09T15:24:26Z
dc.date.available2022-12-09T15:24:26Z
dc.date.issued2022-07-20
dc.descriptionThis article belongs to the Special Issue Emerging Themes in Brucella and Brucellosis.pt_PT
dc.description.abstractBrucellosis, mainly caused by Brucella (B.) melitensis, is associated with a risk of chronification and relapses. Antimicrobial susceptibility testing (AST) standards for B. melitensis are not available, and the agent is not yet listed in the EUCAST breakpoint tables. CLSI recommendations for B. melitensis exist, but they do not fulfill the requirements of the ISO 20776 standard regarding the culture medium and the incubation conditions. Under the third EU Health Programme, laboratories specializing in the diagnostics of highly pathogenic bacteria in their respective countries formed a working group within a Joint Action aiming to develop a suitable method for the AST of B. melitensis. Under the supervision of EUCAST representatives, this working group adapted the CLSI M45 document to the ISO 20776 standard after testing and validation. These adaptations included the comparison of various culture media, culture conditions and AST methods. A Standard Operation Procedure was derived and an interlaboratory validation was performed in order to evaluate the method. The results showed pros and cons for both of the two methods but also indicate that it is not necessary to abandon Mueller–Hinton without additives for the AST of B. melitensis.pt_PT
dc.description.sponsorshipThis research was funded by the EU Health Programme 2014–2020, through the Consumers, Health, Agriculture and Food Executive Agency (CHAFEA, European Commission), the Joint Action EMERGE (CHAFEA n° 677 066) and the Joint Action SHARP (848096-SHARP JA).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMicroorganisms. 2022 Jul 20;10(7):1470. doi: 10.3390/microorganisms10071470pt_PT
dc.identifier.doi10.3390/microorganisms10071470pt_PT
dc.identifier.issn2076-2607
dc.identifier.urihttp://hdl.handle.net/10400.18/8390
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2076-2607/10/7/1470pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBrucella Melitensispt_PT
dc.subjectAntimicrobial Susceptibility Testingpt_PT
dc.subjectInterlaboratory Validationpt_PT
dc.subjectISO 20776 standardpt_PT
dc.subjectCLSIpt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleAdaptation of Brucella melitensis Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Methodpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue7pt_PT
oaire.citation.startPage1470pt_PT
oaire.citation.titleMicroorganismspt_PT
oaire.citation.volume10pt_PT
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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