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Biocompatibility testing and antioxidant properties of cerium dioxide nanoparticles in human nervous system cells

2025-06-06Artigo científico Acesso aberto
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dc.contributor.authorFernández-Bertólez, Natalia
dc.contributor.authorTouzani, Assia
dc.contributor.authorRamos-Pan, Lucía
dc.contributor.authorReis, Ana Teresa
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorLaffon, Blanca
dc.contributor.authorValdiglesias, Vanessa
dc.date.accessioned2026-02-20T16:30:09Z
dc.date.available2026-02-20T16:30:09Z
dc.date.issued2025-06-06
dc.description.abstractCerium dioxide nanoparticles (CeO NP), or nanoceria, are versatile materials with interesting properties for industry and medicine fields, particularly redox properties and catalytic activity. Because of their distinctive features, they have gained high attention in biomedical and pharmacological research to be employed in drug delivery, tissue regeneration, radioprotection, or diagnostic imaging. However, previous works reported that nanoceria may also induce reactive oxygen species (ROS) under certain conditions, leading to cellular stress, cellular damage, or cell death. In this study, the effects of CeO NP on cell viability and morphology as well as their influence on oxidative stress (both oxidant and ROS scavenging capacities) were investigated in nervous system cells (SH-SY5Y neuronal and A172 glial cells) treated with a wide range of CeO NP concentrations (1-100 µg/mL) for several treatment times. Results obtained showed that, despite being stable in time and effectively internalized by both cell types, CeO NP did not produce significant decrease in viability, evaluated by MTT assay, morphological alterations, or intrinsic cell-free ROS, but they generated cellular ROS limited to longer exposure periods. Furthermore, CeO NP demonstrated a certain intrinsic ability to scavenge ROS generated by HO in both tested cell types, more pronounced in neuronal cells. These results confirm the good biocompatibility of nanoceria on human nervous system cells and support further exploring their potential use in biomedicine field, particularly for those therapeutic and diagnostic applications related to the nervous system.eng
dc.description.sponsorshipOpen Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This research was funded by Ministry of Science and Innovation: MCIN/AEI/https://doi.org/10.13039/501100011033 (Grant PID2020-114908GA-I00), Xunta de Galicia (ED431B 2022/16), and FCT—Fundação para a Ciência e Tecnologia, I.P. through UIDB/04750/2020 (https://doi.org/10.54499/UIDB/04750/2020) and LA/P/0064/2020. L.R.-P. was supported by a Ministry of Science and Innovation predoctoral fellowship (grant number FPU2023/03379). Funding for open access charge: Universidade da Coruña/CISUG.
dc.identifier.citationArch Toxicol. 2025 Sep;99(9):3625-3640. doi: 10.1007/s00204-025-04096-y. Epub 2025 Jun 6
dc.identifier.doi10.1007/s00204-025-04096-y
dc.identifier.issn0340-5761
dc.identifier.pmid40478317
dc.identifier.urihttp://hdl.handle.net/10400.18/10976
dc.language.isoen
dc.peerreviewedyes
dc.publisherSpringer
dc.relationEpidemiology Research Unit - Institute of Public Health, University of Porto
dc.relationLaboratory for Integrative and Translational Research in Population Health
dc.relation.hasversionhttps://link.springer.com/article/10.1007/s00204-025-04096-y
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAntioxidant Capacity
dc.subjectCerium Dioxide Nanoparticles
dc.subjectCytotoxicity
dc.subjectGlial cells
dc.subjectNeuronal Cells
dc.subjectOxidative Stress
dc.subjectToxicologia
dc.titleBiocompatibility testing and antioxidant properties of cerium dioxide nanoparticles in human nervous system cellspor
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUIDB/04750/2020
oaire.awardNumberLA/P/0064/2020
oaire.awardTitleEpidemiology Research Unit - Institute of Public Health, University of Porto
oaire.awardTitleLaboratory for Integrative and Translational Research in Population Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04750%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0064%2F2020/PT
oaire.citation.endPage3640
oaire.citation.issue9
oaire.citation.startPage3625
oaire.citation.titleArchives of Toxicology
oaire.citation.volume99
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isProjectOfPublication05f4f337-eeba-4c07-82ed-4202b21618a2
relation.isProjectOfPublication18ca3cb0-ef66-4dd8-b45e-4cb0654cd7e8
relation.isProjectOfPublication.latestForDiscovery05f4f337-eeba-4c07-82ed-4202b21618a2

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